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What are all the advantages of two dimensional electrophoresis?
Two-dimensional electrophoresis allows for separation of proteins based on both charge and size, providing higher resolution compared to one-dimensional electrophoresis. This technique can detect a wider range of proteins in a sample and is useful for identifying post-translational modifications. Additionally, it can be used to compare protein expression levels between different samples.
How Distinguish between latitudes and longitudes?
Lines of latitude run parallel to the equator. Lines of longitude run vertically - passing through both poles.
What is the main difference between protein electrophoresis and nucleic acid electrophoresis?
There are many similarities and differences between protein and DNA electrophoresis.Similarities:PAGE protein and DNA electrophoresis both cause separation by size, creating bands that are viewed by the scientist or a machine. The smallest segments more the fastest due to less friction with the surface of their medium or equipment.The movement of charges through the medium is what causes the DNA or proteins to move. Electrons move from the negative to positive end of the gel or capillary tube.Differences:In PAGE protein electrophoresis, a polyacrylamide gel is used to prevent convection from altering the movement of the proteins. If the proteins are charged, and there is a worry that the charge will affect the mobility of the protein segments, 1% SDS can be added to get rid of the mass/charge issue. This way, only the mass of the segment determines how far it moves. In DNA capillary electrophoresis, the size of the capillary is so small that it does not have room for convection to occur (it is only 20-50 microns wide). Thus, there is no medium in the capillary but DNA itself.In protein electrophoresis, the proteins are often dyed so their movement can be viewed with the naked eye, or a machine. With DNA capillary electrophoresis, DNA strands are made through DNA replication with dNTPs that are fluorescently labeled for the different nucleotides. Each base is labeled a different color. A fine laser lights up the DNA strand in the capillary tube and reads what color fluoresces. This is how the nucleotide is identified.Protein PAGE electrophoresis is used to determine the purity of a protein sample. It can also be used to see how large the chains are that make up a multi-chain protein if a denaturing agent is added. DNA electrophoresis is used to get the order of nucleotides in a DNA sequence. It is done by chopping the DNA sequence into many smaller bits and sequencing them, then putting them back together by lining them up according to sequence overlaps. This is called the "shotgun" method. Protein electrophoresis can figure out the order of about 15-20 amino acids by a similar method, but DNA electrophoresis can get up to 1000 nucleotides (~300 amino acids). DNA electrophoresis is limited by the low probability that the DNA sequence would be cut into a segment greater than 1000 nucleotides.
What are some potential designs for a gel electrophoresis chamber?
a box and a chamber and a lid and a comb A gel electrophoresis can be made two different ways---horizontal or vertical. I'm doing a project with it right now, and honestly, I prefer the horizontal. I don't know how the vertical works, but the horizontal is pretty much a box inside a box with the lid and comb. The bigger box, or your chamber, should be large enough to fit all the wiring on each ends of it, and the smaller box, or the box, in the middle. Your box should have two removable walls on the ends in which the wells are facing, and slits on one end for the comb to go into. You should put the comb in and fill the box up with the gel, and when it dries, you take the comb out and take off both walls. The charge can then run through the walls and to the other side of the chamber.
Which function is performed by both the cytoskeleton and the cell wall?
Both of them support the cell. They help to maintain the shape of the cell
Does the rook in chess attack vertically horizontally or diagonally?
both vertically and horizontally
Do plates move horizontally vertically or both?
Both
Is tgge and dgge same thing?
Temperature Gradient Gel Electrophoresis (TGGE) is a refinement of Denaturing Gradient Gel Electrophoresis (DGGE). Both use the same principles.
A graph cannot be shifted both vertically and horizontally?
Yes, it can.
What letters are horizontal and vertically symmetrical?
The letters that are both horizontally and vertically symmetrical are "H," "I," "O," "X," and "W." These letters maintain their appearance when flipped along both axes. Other letters may be symmetrical along one axis but not the other. For example, "A" is vertically symmetrical but not horizontally.
What are the dimensions of a foursquare court?
The court is 16' X 16' divided evenly both horizontally and vertically into 4 squares
Is the image perceived through the microscope's eyepiece both vertically and laterally inverted?
Yes, the image seen through a microscope's eyepiece is both vertically and laterally inverted. This inversion is a result of the optical system used in microscopes.
Is it ok to have the xbox360 vertical?
Yes, it was designed and advertised to work both horizontally and vertically.
One type of transformation where a geometric figure is slid horizontally vertically or both?
Horizontally
Which require more work lifting a 50-kg sack vertically 2m or lifting a 25-kg sack vertically for 4m?
Both require the same amount of work
What is the color of Lavi's hammer according to both the manga and the anime?
its stripes vertically black and white
An example of symmetry?
The letter A it is vertically symmetric since you can cut it straight down the middle and it will be the same on both sides.
