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What is the function of tank buffer in agarose gel electrophoresis?
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Why is there a need to submerge the agarose gel into a tank of salt solution?
The salt solution serves as a buffer that conducts electricity. Salt is one of the best conductors of electricity.
What is the purpose of gel electrophoresis?
The buffer is the medium through which the current flows. In the electrophoresis chamber, the anode and cathode are separated and the gel is placed between them. In order to close the circuit and generate the voltage which causes the migration, the entire chamber is filled with a conductive buffer. It is actually possible to perform electrophoresis without a buffer; however this requires a specially made electrophoresis chamber. In these chambers the electrodes actually contact the top and bottom of the gel eliminating the need for a conductive buffer to close the circuit.SDS PAGE electrophoresis uses buffer not primarily as a conductor but for holding a desired pH, dissipating heat and providing SDS in excess in the case of denaturing gels. A gel would run without a buffer as the gel itself is a conductor but the currents involved would heat it to the point of decomposition. Also the volume of liquid in a gel does not allow for an adequate pH buffering system. Holding a pH is extremely important for reproducibility especially in native gels as the pH can change the charge on the peptide. It is true some gels do not require buffer but these are rare cases like isoelectric focusing.the primary application of the buffer would be to conduct electricity,to form a closed circuit
What is a buffer tank in wastewater plant?
A buffer tank adds carbon dioxide to wastewater. How much carbon dioxide is added and dissolved in the water affects the pH. In short, a buffer tank is used to adjust the pH of water during treatment.
What are the ratings and certificates for Thomas the Tank Engine and Friends - 1984 Buffer Bother 6-19?
Thomas the Tank Engine and Friends - 1984 Buffer Bother 6-19 is rated/received certificates of: Australia:G
Which fish tank pH buffer is the best Api Wardley or Aquarium pharmaceutics inc?
Api Wardly.
Can salt be used to buffer a fish tank?
No, if they are cold water it will probably kill the fish but hot water, maybe
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this is tha tank where the pressure expand and force to out side. T.J.
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to store fuel
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their is no aerator
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what is the function of aerobic bacteria in the aeration tank
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a small camera on the water tank
Kari is driving across the country The longer she drives after filling her gas tank the more gas she will have to put in next time she fills up?
The amount of gas Kari has to put in her tank is a function of how long she has driven since she last put gas in.
