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Q: What is the importance of surface sterilization in tissue culture?
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What is the simple protocol for banana tissue culture?

in tissue culture should maintain some steps mandatory example explanting,surface sterilization, inoculation , incubation and subculture so their is no any simple protocol for tissue culture especially in banana need to take care still more than others plants because endogenous microbial contamination's will be more in succors so its better to follow specific protocol that should give healty plantlets free from diseases instead of going simple one.


Are tissue culture and plant tissue culture same?

In plant tissue culture, cells of plants are cultured. In tissue culture, cells (of plants, animals, bacteria, etc.) are cultured. Plant tissue culture is just like a subheading under tissue culture


What concentration of alcohol is best to use for sterilization during plant tissue culture. 70 percent alcohol or absolute alcohol?

yes because, 70% alchole make easy contect with suface of the specimen


Dissertation topics in plant tissue culture?

tissue culture of mango se.


How can clone of a plant be produced?

by tissue culture method


What is difference between tissue culture cell culture and organ culture?

to culture any organ , tissue or cell from single cell called cell culture. to grow any tissue or organ from a tissue called tissue cultured. and formation of any organ from source organ is called organ culture. in short according to the source of culture any cultured are named.


Why sterile conditions are needed in tissue culture?

The conditions that allow you to culture tissue are also ideal for the growth of bacteria, which given a chance will devour your tissue culture.


The type of tissue that makes up the surface of the skin?

The type of tissue that makes up the surface of the skin is called stratified squamous epithelium. This tissue is composed of multiple layers of flat cells that provide protection against external stressors and help to prevent dehydration or infection. It is a key component of the epidermis, the outermost layer of the skin.


Why fresh mercuric chloride for tissue culture?

Usuallu 0.1% mercuric chloride is used for surface sterilization. Mercuric chloride reacs with ligth and will loose its activity if it is exposed to light, especially for a longer duration.. So usually fresh mercuric chloride is used (can be stored for a max of 1 or 2 weeks) and dont forget to store in amber coloured bottles, or cover the bottle


What is the importance of tissue matching?

it is important because the wrong tissue can kill you


Why the food making tissue is at the top surface of the leaf?

why is the food-making tissue at the leaf surface why


Which plants are most receptive to tissue culture?

Dicot plants are more receptive to tissue culture due to the presence of cambium.