ewan?
in tissue culture should maintain some steps mandatory example explanting,surface sterilization, inoculation , incubation and subculture so their is no any simple protocol for tissue culture especially in banana need to take care still more than others plants because endogenous microbial contamination's will be more in succors so its better to follow specific protocol that should give healty plantlets free from diseases instead of going simple one.
yes because, 70% alchole make easy contect with suface of the specimen
The conditions that allow you to culture tissue are also ideal for the growth of bacteria, which given a chance will devour your tissue culture.
tissue culture
Cells adhere to the tissue culture flask because the surface of the flask is typically treated with a material (e.g. collagen, gelatin, or fibronectin) that mimics the extracellular matrix, providing a suitable environment for cell attachment. This adhesion is important for cell growth and proliferation, as well as maintaining cell morphology and function in vitro.
in tissue culture should maintain some steps mandatory example explanting,surface sterilization, inoculation , incubation and subculture so their is no any simple protocol for tissue culture especially in banana need to take care still more than others plants because endogenous microbial contamination's will be more in succors so its better to follow specific protocol that should give healty plantlets free from diseases instead of going simple one.
In plant tissue culture, cells of plants are cultured. In tissue culture, cells (of plants, animals, bacteria, etc.) are cultured. Plant tissue culture is just like a subheading under tissue culture
yes because, 70% alchole make easy contect with suface of the specimen
tissue culture of mango se.
by tissue culture method
to culture any organ , tissue or cell from single cell called cell culture. to grow any tissue or organ from a tissue called tissue cultured. and formation of any organ from source organ is called organ culture. in short according to the source of culture any cultured are named.
The conditions that allow you to culture tissue are also ideal for the growth of bacteria, which given a chance will devour your tissue culture.
The type of tissue that makes up the surface of the skin is called stratified squamous epithelium. This tissue is composed of multiple layers of flat cells that provide protection against external stressors and help to prevent dehydration or infection. It is a key component of the epidermis, the outermost layer of the skin.
Usuallu 0.1% mercuric chloride is used for surface sterilization. Mercuric chloride reacs with ligth and will loose its activity if it is exposed to light, especially for a longer duration.. So usually fresh mercuric chloride is used (can be stored for a max of 1 or 2 weeks) and dont forget to store in amber coloured bottles, or cover the bottle
it is important because the wrong tissue can kill you
why is the food-making tissue at the leaf surface why
Dicot plants are more receptive to tissue culture due to the presence of cambium.