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Detection of pentose/ribose sugar in RNA (by performing Bial's test) can be done with Orcinol reagent (0.3% orcinol solution prepared in concentrated HCl).
This method requires the following reagents
1. Orcinol reagent : 6% orcinol in 95% ethanol.
2. Acid reagent : 100 ml. of conc. HCl, 0.5 ml. of 10% FeCl3, 10H2O is added (to an aqueous RNA solution (1 mg. per ml.))
Detection of deoxyribose/deoxypentose sugar in DNA can be identified chemically with the Dische diphenylamine test.
This method requires the following reagents
one gram of purified diphenylamineis dissolved in acetic acid and volume made to 100 ml. with acetic acid. Afterwards, 2.75 ml. of conc. H2SO4 is added for stablization.
Schiff's reagent is another sensitive means , and can be used in a method to demonstrate deoxyribosenucleic acid (DNA) specifically (of detecting aldehydes), in contrast to unstained ribosenucleic acid (RNA). This method is the nucleal reaction of Feulgen and Rossenbeck (called the Feulgen stain or reaction). It is usually done with pararosaniline Schiff solution (pseudo-Schiff reagents), but it works well with some others, including the fluorescent acriflavine solution.
This method requires the following reagents
Hydrochloric acid, 1Normal
Schiff's reagent (made from pararosanilin treated with sulphurous acid)
Light green, 1% aqueous (can be replaced with Fast green FCF)
What else can I help you with?
What macromolecule provides instructions for growth?
Nucleic acids
A nucleic acids is a?
Nucleic acids are polymers of nucleotides.
What macromolecules is made up of nucleic acid?
They are the nucleotides . They make up nucleic acids
Do nucleic acids produce protein?
No. Nucleic acids are the building blocks of protein. There are various types of nucleic acids that form proteins.
Do proteins encode nucleic acids?
No. Nucleic acids encode proteins.
DNA and RNA are 2 examples of what?
Nucleic acids. Dna = Deoxyribonucleic Acid Rna = Ribonucleic Acid
How are nucleic acids and proteins related?
Nucleic acids make proteins.
What are the four macromolecules of life?
The four major macromolecules are proteins, carbohydrates, lipids, and nucleic acids.
What is the name of the macromolecules that makes up DNA?
Nucleic acids.
Is nucleic acids the same as amino acids?
No, nucleic acids are molecules that store and transmit genetic information, such as DNA and RNA, while amino acids are the building blocks of proteins. Proteins are made up of chains of amino acids, not nucleic acids.
When was Nucleic Acids Research created?
Nucleic Acids Research was created in 1974.
Are nucleic acids organic or inorganic compounds?
Nucleic acids are organic compounds.
