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What is the insert capacity of a P1 phage in DNA cloning?
Arvindkataria
You can add maximum 70-100 kb of genetic material in a P1 phage vector.
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∙ 13y ago
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What is the insert capacity of a cosmid vector in DNA cloning?
The insert capacity of a cosmid vector is about 35-45 kb.
What is the insert capacity of a lambda bacteriophage vector in DNA cloning?
We can insert about 5-25 kb sized foreign DNA in a lambda bacteriophage vector.
What is the insert capacity of PACs vectors in DNA cloning?
In a M13 phage we can insert about 1-4 kb of target DNA sequence which is its maximum insert capacity.
What does a phage inject when it attaches to a bacterium?
Dna and phage body
What are phage vectors?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
When phage attaches to a bacteria the phage injects what?
DNA single
What are DNA stuffer regions?
In a bacteriophage that's been modified as a vector for DNA cloning, a "stuffer region" is a sequence of useless DNA that can be removed with specific endonucleases, to prepare a site for insertion of the DNA to be cloned (sometimes called "passenger DNA" - the idea being that the phage is the vehicle).
When the bacteriophage DNA becomes part of the bacterial chromosome?
During phage infection into bacteria, it penetrates phage DNA into bacterium,which will be integrated in to the bacterial genome (chromosome) to replicate and synthesize phage molecules.
What is DNA cloning?
DNA cloning is the production of large number of identical DNA molecules from a single ancestral DNA molecule. It is of two types 1. cell based DNA cloning 2. cell free DNA cloning
In 1952 who showed that DNA is the genetic material of the T2 phage?
Hershey and Chase did a series of classic experiments demonstrating that DNA is the genetic material of the T2 phage.
What is temperate phage superinfection immunity?
c. Repression of the phage genome - A phage coded protein, called a repressor, is made which binds to a particular site on the phage DNA, called the operator, and shuts off transcription of most phage genes EXCEPT the repressor gene. The result is a stable repressed phage genome which is integrated into the host chromosome. Each temperate phage will only repress its own DNA and not that from other phage, so that repression is very specific (immunity to superinfection with the same phage).Reference: http://pathmicro.med.sc.edu/mayer/phage.htm
Name and explain the stages of viral reproduction?
The bacteriophage blinds to the bacterium.The phage DNA enters the host cell.The host DNA is digested, new phage DNA forms, using nucleoleotides from former host DNA.The host cell transcribes and translates the phage DNA, producing phage proteins.Assembly of new is complete. A phage-encoded enzyme causes the cell to lyse.New phage are released to start the cycle again.
