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What is orf in plasmid vector and role in cloning of gene of interest?
ORF stands for Open Reading Frame, which is a sequence of nucleotides that can be translated into a protein. In a plasmid vector, an ORF can be used to clone a gene of interest by inserting the gene sequence into the ORF region, allowing the production of the corresponding protein. The ORF acts as a template for protein synthesis, enabling the expression of the cloned gene in a host organism.
Why do you clone DNA?
Scientists wanted to clone sheep to ultimately learn how to clone a human. But after the failure of cloning the sheep Dolly, many people went against it and cloning against humans is banned in some areas and frowned upon in most.
What are Radioactive strand of RNA or DNA that are complimentary to specific targeted clone fragments and are used to identify a particular fragment in a large population of clones?
These are called probes. They are labeled with a radioactive element and designed to bind specifically to the complementary sequence of the target clone fragment. By detecting the radioactive signal emitted by the probe binding to the target fragment, researchers can identify and locate the specific clone of interest in a large population.
What is an exact duplicate of an organism called?
It is either an identical twin or a clone.
How does the DNA of a clone compare to that of the parent versus how your DNA compares to your parents?
A clone's DNA is genetically identical to that of its parent, as it is created from a single cell of the parent organism, resulting in an exact replication of its genetic material. In contrast, your DNA is a combination of genetic information from both of your parents, inheriting half from each, which introduces variation. This means that while a clone shares the same DNA sequence as its parent, you have a unique genetic makeup that reflects contributions from both your mother and father.
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
What is orf in plasmid vector and role in cloning of gene of interest?
ORF stands for Open Reading Frame, which is a sequence of nucleotides that can be translated into a protein. In a plasmid vector, an ORF can be used to clone a gene of interest by inserting the gene sequence into the ORF region, allowing the production of the corresponding protein. The ORF acts as a template for protein synthesis, enabling the expression of the cloned gene in a host organism.
Why did Michael Jackson want to clone himself?
He never wanted to clone himself.
What is pcDNA?
pcDNA is DNA vector used to clone recombinant DNA sequences for the expression of proteins of interest in mammalian cells.
Should you clone people or things?
you should because if you need a transplant, your clone could donate blood or an organ to you.
How would the DNA of your clone compare to the DNA of you?
Technically, it should be identical. The DNA should be exactally the same. This is what makes it a clone.
Should you use phase 1 clone or phase 2 clone?
There is little to no difference.
How do you clone Pokemon in Pokemon Y?
When you're trading with someone, right when it says, please wait after you traded the Pokemon you wanted to clone, turn your game off.
How do i catch three regis in emerald?
get and clone masterballs then do the stuff they tell you to and you should be good get and clone masterballs then do the stuff they tell you to and you should be good
Is it okay to clone farm animals?
NO its not OK to clone animals is it a genetically bonded animal and should not be tampered with x
Significance of vector in DNA recombinant technology?
Vector are plasmid DNA, act as a molecular vehicles to carry genes or DNA of interest. In rDNA technology vectors used to clone the gene by ligation. This chimeric DNA or plasmid can be propagated in E.coli as the vector carries its own origin of replication. Expression plasmid vectors can be used to produce proteins from the gene of interest.
What should be invented to make the human world better?
a clone of me
