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Q: What is the minimum requirement for a positive reaction in biuret test?
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What color will a peptide bond be after using the Biuret test?

in biuret test the presence of peptide bonds gives a violet color. but it should be noted that a minimum of two peptide bonds should be present in the protein to get a positive answer to this test.


What is the color of biruet solution?

The biuret solution used in the biuret test for peptide bonds is blue in the absence of peptide bonds or biuret which also results in a positive result. A positive result for peptide bonds or biuret is apparent as the biuret solution turns purple/violet in color.


Does pepsin test positive in biuret test?

No


Will glutamic acid give a positive biuret test?

Amino acids doesn't react with the biuret reagent.


How many of these chemical groupings must be present in a molecule to give a positive biuret test?

you should have at least 2 of these,to obtain a positive result in Biuret's test. because biuret test is a test used to detect if there is peptide linkages.


Does phenol give a positive biuret test?

No. The Biuret test only gives a positive result if a peptide bond exists. Phenol doesn't have a peptide bond, so it will not give a positive test.


What color is a positive test for protein?

A positive test, when using Biuret reagent, would be purple-ish


Is biuret test suitable to test the presence of proteins in the urine?

Yes, the biuret test will indicate the presence of peptides. If it turns violet, it is a positive result.


How do you tell if a substance contains protein by using biuret test?

If Biuret Reagent is related to the presence of Protein macromolecules, then a positive result will change it's colour from blue to violet.


What the uses of sodium hydroxide in the Biuret test?

To alkaline the medium so that the reaction can take place


What is the essense of adding water to a biuret reagent do you have to add water to the reaction?

water turns blue


Would albumin give a positive biuret test?

Hey. okay.. the biuret test is a test for peptide bonds. when CuSO4 is added to KOH, an alkaline solution, nitrogen atoms from the peptide bond forms a purple compound with the Cu2+ ions. hope this helps! read it in a text book.