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A positive reaction for albumin in the biuret test occurs because albumin contains peptide bonds, which the biuret reagent detects. The reagent contains copper ions that bind to these peptide bonds, resulting in a color change, typically to violet or purple. This reaction indicates the presence of proteins, specifically those containing multiple peptide bonds, like albumin. Therefore, a positive biuret test confirms the presence of protein in the sample.

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Hey. okay.. the biuret test is a test for peptide bonds. when CuSO4 is added to KOH, an alkaline solution, nitrogen atoms from the peptide bond forms a purple compound with the Cu2+ ions. hope this helps! read it in a text book.


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