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What is the molar absorptivity of Cu2 plus at 620 nm given that the cuvettes used in lab had a pathlength of 1.00 cm with a concentration verse absorbtivity curve of y equals .727x plus .0557?
Wiki User
∙ 14y ago
.727/g*M, slope is molar absorbtivity
Wiki User
∙ 14y ago
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Identify function of each of the cuvettes?
hff
Why do you use quartz cuvette rather than using glass cuvettes?
Glass will absorb radiation below 320nm whereas quartz will allow transmission of the UV wavelengths. So use glass cuvettes for wavelengths in the visible range from 380nm to 780nm and quartz cuvettes for wavelengths below 380nm.
How is your blank cuvette different from your other cuvettes?
It is your control. Your baseline reading.
What are the precautions to be taken while using UV spectrophotometer?
One should not touch the optical assembly with bear hands. Optical surface of cuvettes should not be touched with fingers to avoid erratic reading. Always ensure effectiveness of silica gel kept in instrument. Preserve the instrument from dust particles. Never wash the cuvettes with chromic acid. Wash the cuvette with the distilled water and then with methanol before storage. Check the calibration status before using the instrument and ensure the timely calibration. Do not overfill the cuvettes. Perform the visual inspection of cuvettes for suitability.
Why are cuvettes that are used in the 200-400nm range made of silica?
A cuvette is a very small tube that is sealed at one end and made of plastic, glass, or fused quartz. The reason why the cuvettes that are used in the 200-400 nm range are made of silica is because of the shifts in the chemical equilibria.
What has the author Radu Mavrodineanu written?
Radu Mavrodineanu has written: 'Bibliography on flame spectroscopy' 'Standard quartz cuvettes for high accuracy spectrophotometry' -- subject(s): Cuvettes (Optical instrument), Spectrophotometer, Standards 'Summary of the biological and botanical standards issued by the National Bureau of Standards' -- subject(s): Biochemistry, Biological apparatus and supplies, Biological specimens, Botanical apparatus, Botanical chemistry, Handbooks, manuals, Standards
Why wasn't dpip added to cuvette 1?
Because it was the control. Note how all other cuvettes had DPIP. We did not really know what dpip's effect on the % light transmittance was, by adding a ontrol, we could not compare and contrast.
What reasons can you give for the difference in the percentage of transmittance between the live chlorplats that were incubated in the light and those that were kept in the dark?
The percent transmittance grew to steadily higher numbers as the experiment progressed because the light reaction was able to occur. However, the dark cuvettes had stable levels of transmittance because light is necessary to excite electrons, which, in turn, reduces the DPIP.
What are the advantages and disadvantages of Bradford protein assay?
Advantages: Compatible with most buffers, reagents and preparations. One step procedure that is sensitive and accurate. Disadvantages: Non linear correlation over large range of protein concentrations. The dye binds to surface of cuvettes so calibration curves need to be made every time. Also the dye binds to test tubes so washing after use immediate use is a must. High concentration detergents can interfere with assay.
What is a cuvette and how is it used?
A cuvette is, basically, a glorified test tube for use in a spectrometer. They're sometimes square in cross section to avoid refraction artifacts. Depending on what part of the spectrum is under consideration, they may be made of quartz, though plastic and glass cuvettes do exist. You put a liquid sample into it then put the cuvette into the instrument. The beams inside of the instrument can 'see through' the quartz and 'read' your sample.
What are some eight letter words with 1st letter C and 3rd letter V and 5th letter T and 6th letter T?
According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 2 words with the pattern C-V-TT--. That is, eight letter words with 1st letter C and 3rd letter V and 5th letter T and 6th letter T. In alphabetical order, they are: cavettos cuvettes
What are some eight letter words with 1st letter C and 2nd letter U and 3rd letter V and 5th letter T and 7th letter E?
According to SOWPODS (the combination of Scrabble dictionaries used around the world) there are 1 words with the pattern CUV-T-E-. That is, eight letter words with 1st letter C and 2nd letter U and 3rd letter V and 5th letter T and 7th letter E. In alphabetical order, they are: cuvettes
