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I. Transform bacteria with recombinant DNA molecule

II. Cut the plasmid DNA using restriction enzymes

III. Extract plasmid DNA from bacterial cells

IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments

V. Use ligase to seal plasmid DNA to nonplasmid DNA

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13y ago
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14y ago

replicate and divide evenly

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Q: What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
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