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Blood and Drabkins Reagent (RBCs lysed and Hgb released)
Hgb oxidized to methoglobin by K ferricyanide
Resulting pigment converted to stable cyanmethoglobin by Kcyanide
Absorption of pigment measured photometrically at 540nm
Concentration of Hgb is directly proportional to optical density of solution
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What is the principle of the cyanmethemoglobin method?
Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.
What is the significance of the development of the cyanmethemoglobin method?
The development of the cyanmethemoglobin method is significant in hematology and clinical medicine because it allows for accurate measurement of hemoglobin levels in blood samples. This method is considered more reliable than older techniques and has become a standard in blood testing due to its precision and reproducibility. It is particularly important for diagnosing and monitoring conditions such as anemia and other blood disorders.
Hb estimation by drabkin's method?
Drabkin's method is a commonly used laboratory technique to estimate hemoglobin levels in the blood. It involves lysing the red blood cells to release hemoglobin and then measuring the absorbance of the hemoglobin-cyanmethemoglobin complex at a specific wavelength to determine the concentration of hemoglobin in the sample. This method provides a quick and accurate assessment of hemoglobin levels, which is crucial for diagnosing conditions like anemia.
What does procedure mean in a experiment?
A procedure in an experiment refers to the set of steps or instructions that are followed to conduct the experiment. It outlines how the variables will be manipulated and measured in order to test a hypothesis and obtain results. Following the procedure accurately is crucial for ensuring the reliability and validity of the experiment.
How do you write an experimental procedure effectively?
To write an effective experimental procedure, clearly outline the steps in a logical order, include all necessary materials and equipment, provide specific measurements and variables, and ensure the procedure is detailed enough for someone else to replicate the experiment accurately.
Disadvantages using cyanmethemoglobin method?
three disadvantages cyanmethemoglobin
Advantages and disadvantages using cyanmethemoglobin method?
The Cyanmethemoglobin method is a commonly used laboratory procedure for the estimation of hemoglobin in blood. This method has several advantages and disadvantages. Advantages: Accuracy: The Cyanmethemoglobin method is considered very reliable and is often used as a reference method. Stability: The Cyanmethemoglobin solution is stable, and readings can be taken at a later time without significant alteration. Interference: It is less susceptible to turbidity and lipemia interference as compared to other methods. Disadvantages: Toxicity: The reagent used, Drabkin's solution, contains potassium cyanide, which is toxic and needs to be handled with care. Time: The reaction time to obtain cyanmethemoglobin is relatively longer compared to other methods. Interference: The method can overestimate hemoglobin if a large number of white cells, such as in leukemias, are present. In conclusion, while the Cyanmethemoglobin method has its advantages, including accuracy and stability, it also has some disadvantages such as toxicity of the reagent used and potential interference in certain conditions. It's crucial to carefully consider these factors before deciding on a method for hemoglobin estimation.
What is the principle of the cyanmethemoglobin method?
Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.
Which form of hemoglobin cannot be measured using the cyanmethemoglobin method?
sulphaemoglobin shb
What is the significance of the development of the cyanmethemoglobin method?
The development of the cyanmethemoglobin method is significant in hematology and clinical medicine because it allows for accurate measurement of hemoglobin levels in blood samples. This method is considered more reliable than older techniques and has become a standard in blood testing due to its precision and reproducibility. It is particularly important for diagnosing and monitoring conditions such as anemia and other blood disorders.
Hb estimation by drabkin's method?
Drabkin's method is a commonly used laboratory technique to estimate hemoglobin levels in the blood. It involves lysing the red blood cells to release hemoglobin and then measuring the absorbance of the hemoglobin-cyanmethemoglobin complex at a specific wavelength to determine the concentration of hemoglobin in the sample. This method provides a quick and accurate assessment of hemoglobin levels, which is crucial for diagnosing conditions like anemia.
Procedure of valuation of goodwill?
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What is an operators?
A procedure, particularly a surgical procedure or military procedure.
Which are the Methods for hemoglobin estimation?
Hemoglobin estimation is the measuring of hemoglobin levels that are broken into three categories. Visual methods are Sahil, Dares, Hadens, Wintrobes, Haldanes, Tallquists method. Second to consider are the gasometric method, single to the Van Slyke method. Lastly the spectrophotometric method oxyhemoglo, and the cyanmethemoblobin method.
What is procedure and the answer?
s a procedure
What is the difference between a diagnostic procedure and a therapeutic procedure?
diagnostic procedure discovers whats wrong, and therapeutic procedure treats whats wrong
What is the difference between a therapeutic procedure and a diagnostic procedure?
diagnostic procedure discovers whats wrong, and therapeutic procedure treats whats wrong
