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Blood and Drabkins Reagent (RBCs lysed and Hgb released)
Hgb oxidized to methoglobin by K ferricyanide
Resulting pigment converted to stable cyanmethoglobin by Kcyanide
Absorption of pigment measured photometrically at 540nm
Concentration of Hgb is directly proportional to optical density of solution
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What is the principle of the cyanmethemoglobin method?
Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.
What is the significance of the development of the cyanmethemoglobin method?
ewan ko sayo,assignment mo yan eh...
Is copper T insertion is painful procedure?
The insertion of a copper T can be a painful procedure. Within a few months after the procedure, the pain from the heavy periods will subside as the body gets used to the copper T.
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Disadvantages using cyanmethemoglobin method?
three disadvantages cyanmethemoglobin
Advantages and disadvantages using cyanmethemoglobin method?
The Cyanmethemoglobin method is a commonly used laboratory procedure for the estimation of hemoglobin in blood. This method has several advantages and disadvantages. Advantages: Accuracy: The Cyanmethemoglobin method is considered very reliable and is often used as a reference method. Stability: The Cyanmethemoglobin solution is stable, and readings can be taken at a later time without significant alteration. Interference: It is less susceptible to turbidity and lipemia interference as compared to other methods. Disadvantages: Toxicity: The reagent used, Drabkin's solution, contains potassium cyanide, which is toxic and needs to be handled with care. Time: The reaction time to obtain cyanmethemoglobin is relatively longer compared to other methods. Interference: The method can overestimate hemoglobin if a large number of white cells, such as in leukemias, are present. In conclusion, while the Cyanmethemoglobin method has its advantages, including accuracy and stability, it also has some disadvantages such as toxicity of the reagent used and potential interference in certain conditions. It's crucial to carefully consider these factors before deciding on a method for hemoglobin estimation.
What is the principle of the cyanmethemoglobin method?
Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.
Which form of hemoglobin cannot be measured using the cyanmethemoglobin method?
sulphaemoglobin shb
What is the significance of the development of the cyanmethemoglobin method?
ewan ko sayo,assignment mo yan eh...
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