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Is lowry's protein estimation an invasive method?
No, Lowry's protein estimation method is not invasive. It is a biochemical method used to quantify the total protein concentration in a sample based on the reaction of proteins with specific reagents. It does not involve physical penetration or manipulation of the sample.
How is the Gerber method of fat estimation done?
The Gerber method of fat estimation involves mixing milk with sulfuric acid and centrifuging it to separate the fat. The fat layer is then measured and compared to a standard to determine the fat content of the milk. It is a widely used method in dairy industry for accurate estimation of fat content in milk.
What are experimental errors which effect Hb level in sahlis experiment?
Some potential experimental errors that could affect hemoglobin (Hb) levels in Sahli's method for estimating Hb include inaccurate measurement of blood volume, improper mixing of blood with reagents, and variability in interpreting color changes due to human subjectivity. These errors can lead to inaccurate readings and affect the reliability of the Hb level determination. It is important to follow the protocol carefully to minimize such errors.
Why protein estimation is done at 660 nanometer in folin lowry method?
Protein estimation in the Folin-Lowry method is done at 660 nanometers because this wavelength corresponds to the highest absorption peak of the complex formed between proteins and the Folin-Lowry reagent. This wavelength is ideal for accurately measuring the concentration of proteins present in a sample based on the colorimetric reaction that occurs.
Why do you wait after adding the blood to hcl for ten minutes in sahli's method of hb?
Waiting for 10 minutes after adding blood to HCl in Sahli's method allows for complete lysis of red blood cells and release of hemoglobin. This step ensures that hemoglobin is in its free form and stable for accurate measurement.
What is the principle of hemoglobin estimation using cyanmethemoglobin method?
what are the various methods of the Hb estimation???/kindly tell me the senstivity of the methods. i know only one method sahli hemoglobinometer(acid hematin) method. I have enumerated few Sahli - Adam's Method Dare's method Haden's method Wintrobe's method Haldane's method Talliquist method Gasometric method Spectrophotometric method Automated & non-automated hemolglobinometry Other methods alkaline hematin method specific gravity comparator method
Why hcl is used in Hb estimation?
HCl (hydrochloric acid) is used in the estimation of hemoglobin (Hb) because it helps to release hemoglobin from red blood cells, making it more accessible for measurement. It also helps to remove other interfering substances, ensuring a more accurate estimation of hemoglobin levels.
Why Hydrochloric acid is used for hemoglobin estimation?
to convert the Hb to acid-hematin which is a brown coloured complex.
What is front end estimation?
Front end estimation- An estimation method in which the front digits are added or subtracted
What is a method for math in a estimation?
round or rounding
What is an invasive method of protein estimation?
gand mara
Can one cost estimation method be used in combination with another method?
Yes. True
One cost estimation method can be used in combination with another method.?
Yes. True
Can one cost estimation method can be used in combination with another method?
Yes. True
What are the methods of estimation of reducing sugar?
by comparing the colours or the amount of precipitate
What is the method of estimation using a representative section of a whole?
sampling
What is a method of estimation using a representative section of the whole?
sampling
