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What is the principle of the cyanmethemoglobin method?
Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.
What is the procedure of cyanmethemoglobin?
Blood and Drabkins Reagent (RBCs lysed and Hgb released) Hgb oxidized to methoglobin by K ferricyanide Resulting pigment converted to stable cyanmethoglobin by Kcyanide Absorption of pigment measured photometrically at 540nm Concentration of Hgb is directly proportional to optical density of solution
Hb estimation by drabkin's method?
Drabkin's method is a commonly used laboratory technique to estimate hemoglobin levels in the blood. It involves lysing the red blood cells to release hemoglobin and then measuring the absorbance of the hemoglobin-cyanmethemoglobin complex at a specific wavelength to determine the concentration of hemoglobin in the sample. This method provides a quick and accurate assessment of hemoglobin levels, which is crucial for diagnosing conditions like anemia.
What is significance of Zeisel's method?
zeisel method is used for the estimation of alkoxy group in ether. ether is treated with conc. HI and heated. alkyl iodide thus formed is treated with alcoholic silver nitrate solution . AgI is precipitated. it is washed and dried. from the amount of AgI alkoxy group is estimated.
What is the significance of the Gaussian modredundant method in computational chemistry?
The Gaussian modredundant method is significant in computational chemistry because it allows for more accurate calculations of molecular structures and properties. This method helps to reduce computational costs while maintaining high levels of accuracy, making it a valuable tool for studying complex chemical systems.
Disadvantages using cyanmethemoglobin method?
three disadvantages cyanmethemoglobin
What is the principle of the cyanmethemoglobin method?
Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.
Advantages and disadvantages using cyanmethemoglobin method?
The Cyanmethemoglobin method is a commonly used laboratory procedure for the estimation of hemoglobin in blood. This method has several advantages and disadvantages. Advantages: Accuracy: The Cyanmethemoglobin method is considered very reliable and is often used as a reference method. Stability: The Cyanmethemoglobin solution is stable, and readings can be taken at a later time without significant alteration. Interference: It is less susceptible to turbidity and lipemia interference as compared to other methods. Disadvantages: Toxicity: The reagent used, Drabkin's solution, contains potassium cyanide, which is toxic and needs to be handled with care. Time: The reaction time to obtain cyanmethemoglobin is relatively longer compared to other methods. Interference: The method can overestimate hemoglobin if a large number of white cells, such as in leukemias, are present. In conclusion, while the Cyanmethemoglobin method has its advantages, including accuracy and stability, it also has some disadvantages such as toxicity of the reagent used and potential interference in certain conditions. It's crucial to carefully consider these factors before deciding on a method for hemoglobin estimation.
Which form of hemoglobin cannot be measured using the cyanmethemoglobin method?
sulphaemoglobin shb
What is the significance of scientific method?
what is the significance of scientific method?what is the significance of scientific method?
What is the procedure of cyanmethemoglobin?
Blood and Drabkins Reagent (RBCs lysed and Hgb released) Hgb oxidized to methoglobin by K ferricyanide Resulting pigment converted to stable cyanmethoglobin by Kcyanide Absorption of pigment measured photometrically at 540nm Concentration of Hgb is directly proportional to optical density of solution
Which are the Methods for hemoglobin estimation?
Hemoglobin estimation is the measuring of hemoglobin levels that are broken into three categories. Visual methods are Sahil, Dares, Hadens, Wintrobes, Haldanes, Tallquists method. Second to consider are the gasometric method, single to the Van Slyke method. Lastly the spectrophotometric method oxyhemoglo, and the cyanmethemoblobin method.
What is the principle of hemoglobin estimation using cyanmethemoglobin method?
what are the various methods of the Hb estimation???/kindly tell me the senstivity of the methods. i know only one method sahli hemoglobinometer(acid hematin) method. I have enumerated few Sahli - Adam's Method Dare's method Haden's method Wintrobe's method Haldane's method Talliquist method Gasometric method Spectrophotometric method Automated & non-automated hemolglobinometry Other methods alkaline hematin method specific gravity comparator method
What is the definition of method development by hplc?
Method development is a process amenable to continuous improvement
What is the significance of theatre to the history and development of japan?
The significance of of theatre to the history and development of Japan is to entertain and perform elements in other activities.
What is adhoc system development method?
this is the development of a system as at when it is required.
What is the significance of McCullough Maryland in the development of the federal system?
The significance of McCullough V Maryland contain two things, which are taxes and spend. This is in the development of the federal system.
