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Disadvantages using cyanmethemoglobin method?

three disadvantages cyanmethemoglobin


Advantages and disadvantages using cyanmethemoglobin method?

The Cyanmethemoglobin method is a commonly used laboratory procedure for the estimation of hemoglobin in blood. This method has several advantages and disadvantages. Advantages: Accuracy: The Cyanmethemoglobin method is considered very reliable and is often used as a reference method. Stability: The Cyanmethemoglobin solution is stable, and readings can be taken at a later time without significant alteration. Interference: It is less susceptible to turbidity and lipemia interference as compared to other methods. Disadvantages: Toxicity: The reagent used, Drabkin's solution, contains potassium cyanide, which is toxic and needs to be handled with care. Time: The reaction time to obtain cyanmethemoglobin is relatively longer compared to other methods. Interference: The method can overestimate hemoglobin if a large number of white cells, such as in leukemias, are present. In conclusion, while the Cyanmethemoglobin method has its advantages, including accuracy and stability, it also has some disadvantages such as toxicity of the reagent used and potential interference in certain conditions. It's crucial to carefully consider these factors before deciding on a method for hemoglobin estimation.


What is the procedure of cyanmethemoglobin?

Blood and Drabkins Reagent (RBCs lysed and Hgb released) Hgb oxidized to methoglobin by K ferricyanide Resulting pigment converted to stable cyanmethoglobin by Kcyanide Absorption of pigment measured photometrically at 540nm Concentration of Hgb is directly proportional to optical density of solution


What is the principle of the cyanmethemoglobin method?

Colorimetric method where blood is mixed with solution containing potassium ferricyanide and potassium cyanide. Potassium ferricyanide oxidizes iron to form methemoglobin and potassium cyanide then combines with methemoglobin to form cyanmethemoglobin.


Which form of hemoglobin cannot be measured using the cyanmethemoglobin method?

sulphaemoglobin shb


What is the significance of the development of the cyanmethemoglobin method?

The development of the cyanmethemoglobin method is significant in hematology and clinical medicine because it allows for accurate measurement of hemoglobin levels in blood samples. This method is considered more reliable than older techniques and has become a standard in blood testing due to its precision and reproducibility. It is particularly important for diagnosing and monitoring conditions such as anemia and other blood disorders.


Which are the Methods for hemoglobin estimation?

Hemoglobin estimation is the measuring of hemoglobin levels that are broken into three categories. Visual methods are Sahil, Dares, Hadens, Wintrobes, Haldanes, Tallquists method. Second to consider are the gasometric method, single to the Van Slyke method. Lastly the spectrophotometric method oxyhemoglo, and the cyanmethemoblobin method.


What is the principle of hemoglobin estimation using cyanmethemoglobin method?

what are the various methods of the Hb estimation???/kindly tell me the senstivity of the methods. i know only one method sahli hemoglobinometer(acid hematin) method. I have enumerated few Sahli - Adam's Method Dare's method Haden's method Wintrobe's method Haldane's method Talliquist method Gasometric method Spectrophotometric method Automated & non-automated hemolglobinometry Other methods alkaline hematin method specific gravity comparator method


In the method of contraception chemicals are used to kill or immobilize sperm?

This method is known as spermicide. Spermicides are substances that contain chemicals that immobilize or kill sperm, thus preventing them from reaching and fertilizing an egg. Spermicides can come in various forms such as foam, gel, cream, or suppositories and are often used in conjunction with other contraceptive methods like condoms.


What comprises Lloyd's reagent?

It is a hydrated aluminium silicate that is used to improve sensitivity and specifity of Jaffe's method. It is used to adsorb creatinine in acidic condition and hence seperate it from other chromogens.


What is wet chemical method?

The wet chemical method is a technique used in chemical analysis that involves dissolving a sample in a liquid reagent to perform a chemical reaction. It is commonly used for qualitative and quantitative analysis of chemical compounds in various industries such as pharmaceuticals, environmental monitoring, and food testing. The results obtained from the wet chemical method help in determining the composition and properties of a sample.


A colormertric method calls for the use of 0.1ml serum 5ml of reagent and 4.9ml of water what is the final dilution?

1:5