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Splicing
Transgenic - Refers to an organism containing one or more deliberately inserted genes from another species. Examples are bacteria containing the gene for human insulin and plants that contain the gene for a naturally occurring insecticide.
You would need to use Gene splicing to insert a foreign gene into an organism.
Genetic Engineering
Gene transfer between cells generally consists of the following steps: 1. Isolating the gene of interest - Here, the gene which has to be transferred has to be isolated from the genome of the source (or host) organism. 2. Splicing the gene if interest into a plasmid. Splicing is a process wherein a foreign strand of DNA (the gene of interest) is inserted into a loop of DNA called a plasmid. The plasmid DNA is cut open to form a linear fragment. The gene of interest is then attached to the plasmid DNA. The plasmid DNA is converted back into the loop form with the help of an enzyme called DNA ligase. 3. Gene amplification: Here, the plasmid containing the gene of interest is amplified. Which means, many copies of the plasmid containing DNA are created through a process called the polymerase chain reaction. 4. Transfection: This is the final step wherein the plasmid containing DNA is inserted into the recipient organism. Sometimes the foreign DNA remains within the plasmid and is able to express protein. Other times, the gene of interest can be engineered to contain a sequence called the recombination sequence which will allow it to integrate (or join) the host genome through a process called homologous recombination. By the method described above, a foreign gene is removed from one organism and inserted into another. If the gene of interest is integrated into the host of the recipient organism, copies of it are made every time the host cells divide.
Bacteria is often the organism a gene is removed from. The gene is inserted into another species, often corn, soy, papaya, canola, etc.
Splicing
Transgenic - Refers to an organism containing one or more deliberately inserted genes from another species. Examples are bacteria containing the gene for human insulin and plants that contain the gene for a naturally occurring insecticide.
You would need to use Gene splicing to insert a foreign gene into an organism.
isolating and then transferring a gene into the DNA of another organism.
Genetic Engineering
genetic engineering is the process of transferring a gene from the dna of one organism to another organism. in order to produce an organism with desired traits.
A transgenic organisms has one or more genes from another organism inserted into its genome.Transgenic bacteria with the gene for human insulin make human insulin that is used to treat people with Diabetes.
In genetic engineering, a gene is removed from one organism and inserted into an organism of an totally different species.
development
A genetically modified organism results from a gene being removed from one organism and artificially inserted into an organism of a totally different species. This is a simplified explanation and the process is done in a lab.
Gene transfer between cells generally consists of the following steps: 1. Isolating the gene of interest - Here, the gene which has to be transferred has to be isolated from the genome of the source (or host) organism. 2. Splicing the gene if interest into a plasmid. Splicing is a process wherein a foreign strand of DNA (the gene of interest) is inserted into a loop of DNA called a plasmid. The plasmid DNA is cut open to form a linear fragment. The gene of interest is then attached to the plasmid DNA. The plasmid DNA is converted back into the loop form with the help of an enzyme called DNA ligase. 3. Gene amplification: Here, the plasmid containing the gene of interest is amplified. Which means, many copies of the plasmid containing DNA are created through a process called the polymerase chain reaction. 4. Transfection: This is the final step wherein the plasmid containing DNA is inserted into the recipient organism. Sometimes the foreign DNA remains within the plasmid and is able to express protein. Other times, the gene of interest can be engineered to contain a sequence called the recombination sequence which will allow it to integrate (or join) the host genome through a process called homologous recombination. By the method described above, a foreign gene is removed from one organism and inserted into another. If the gene of interest is integrated into the host of the recipient organism, copies of it are made every time the host cells divide.