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Human insulin is made with bacteria through recombinant DNA. It introduces a copy of human insulin DNA to a host cell, which then produces human insulin.

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Q: What is the process of making human insulin with bacteria?
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Is the insulin made by E.coli better or worse than insulin made by human?

The insulin that we engineer bacteria to make (E.colidoes not naturally make insulin) is identical to human insulin.


What kind of organisms produce human insulin?

Bacteria


What kind of organism is to produce human insulin?

*A+*bacteria


How can bacteria be used to help people that are diabetic?

Scientists have put some bacteria to work making medicines and other substances. The first medicine-producing bacteria were made in the 1970s. By manipulating the bacteria's genetic material, scientists engineered bacteria to produce human insulin. Although healthy people can make their own insulin, those with some types of diabetes cannot. Many people with diabetes need to take insulin daily. Thanks to bacteria's fast rate of reproduction, large number of insulin-making bacteria can be grown in huge vats. The human inlusin they produce is then perified and made into medicine. CREDITS FROM: NORTH CAROLINA 8TH GRADE PRENTICE HALL SCIENCE EXPLORER Pg.225


What is synthetic human insulin?

Insulin is a hormone secreted from the pancreas in response to high blood sugar levels. Since it is a hormone it is a protein. Proteins can be synthesized with the help of bacteria in a process called pcr. Synthesizing insulin would not be easy at home. Hormones are commonly obtained from animals such as the pig.


How is commercial insulin prepared?

Standard insulin is an extract taken from the pancreases of cattle from slaughterhouses. However not all diabetics respond well to animal insulin. Genetic engineering has provided a way to manufacture actual human insulin for diabetics that can't use animal insulin. The human insulin gene (edited to remove the introns, which bacteria can't process correctly) is inserted into the chromosome of a bacterium and the bacteria are allowed to grow. Once they have a pure culture of the modified bacteria they are grown in large fermentation tanks. The bacteria produce and secrete the human insulin into the liquid in the tank and as this liquid is circulated to replenish the food for the growing bacteria the insulin is extracted. Some additional processing is needed to perform some steps that the bacteria can't do, the end result is true human insulin.


How is bacteria used to make insulin?

Human plasmids introduced into the bacteria stimulate insulin production. A special enzyme is used to cut out the insulin gene from a human cell. It is attached to a bacterial chromosome which is also split open by an enzyme. The gene is then transferred into a bacterial cell. The gene makes the bacterial cell produce insulin.


What kind of organism is used to produce human insulin?

*A+*bacteria


Which type of DNA technology is used to cause bacteria to produce human insulin?

The type of DNA technology used to cause bacteria to produce human insulin is recombinant DNA technology. In this process, the gene for human insulin is inserted into the genome of a bacterium, such as Escherichia coli (E. coli), using techniques such as restriction enzymes and ligase enzymes. Once the gene is inserted, the bacterium is then able to produce human insulin, which can be purified and used for medical purposes. This technology has revolutionized the production of insulin, making it more accessible and affordable for people with diabetes. Recombinant DNA technology has also been used to produce many other human proteins, such as growth hormone and blood clotting factors, with great success.


Which statement best describes human insulin that is produced by by genetically engineered bacteria?

Genetically engineered insulin may have fewer side effects than the insulin previously extracted from the pancreases of other animals. This is because genetic engineering inserts human genes such as the gene for insulin production into the DNA of bacteria. As a result, the bacteria that produces human insulin, when used by diabetics, should produce fewer side effects.


Is diabetes insulin made in a pig cow human or bacteria?

Diabetes insulin is found in all four subjects. However, recombinant bacteria are primarily used as a main source of insulin for diabetic subjects.


How bacteria can make human insulin?

Yes, bacteria are used to make insulin. First restriction enzymes cut a human DNA strand where the gene to make insulin is located. Then, that fragment of human DNA is inserted into a bateria plasmid that reproduces and as it reproduces it creates more insulin.