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What is the protocol for culture of fungi on rice starch agar media?
Wiki User
∙ 10y ago
* Prepare a Rice Starch Agar Media (RSA)
* Sterilize the media , glasswares , pipette etc. by autoclave at 121 degree C temp. and 15lbs pressure for 15 mins.
* take the media and other things to the aseptic chamber.
* Pour the media in the petri plate.
* Allow it to solidify.
* Inoculate the sample with the help of inoculating loop on the media.
* Incubate it at RT for 3-7 days for fungal growth.
* Obeserve for the shape, size, appearance of the colonies.
Wiki User
∙ 10y ago
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