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I think the Question should be " What is the use of a dye in electrophoresis?" to be more specific, The normal dyes that are incorporated into the sample loading buffer in case of agarose gel is bromophenol blue and xylene cyanol.These are used to track the movement of the sample, so that we can stop the electrophoresis when the dye front is nearly 80%(can vary depending on the molecular weight of the sample) away from the well.
In case of polyacrylamide gel electrophoresis the dye has many functions:
gives density to the sample so that it sits properly in the well.
SDS binds to the amino acid and makes it anionic, so that the movement of the sample is based on molecular weight, if not the charge of the protein will also influence the movement.
it also helps us to track the movement of the sample.
DTT or mercaptoethanol helps in the breaking of cysteine bonds..
That's it.. But the question is too broad and can be specific!!!!!!!!!1
Wiki User
∙ 12y ago
Wiki User
∙ 12y agogo to wikipedia and it will tell you the answer or you can ask your science teacher or local gp..
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What is the purpose of using a biological stain when microscopically examining cellular components?
the purpose of using stain is to highlight specific areas or parts of the cell. different cell structures become visible under different stain colors.
What is the purpose of methyl alcohol in Leishman's stain?
It acts as a fixative and fixes the cells
What is a technique for sorting DNA fragments by length?
it is called " electrophoresis"
What is the purpose of immunoelectrophoresis?
Immunoelectrophoresis aids in the diagnosis and evaluation of the therapeutic response in many disease states affecting the immune system. It is usually requested when a different type of electrophoresis, called a serum protein.
What is the purpose of adding Lugol's iodine to potato slide?
To stain the starch in the leucoplasts so they are visible
What is the purpose of using a biological stain when microscopically examining cellular components?
the purpose of using stain is to highlight specific areas or parts of the cell. different cell structures become visible under different stain colors.
Purpose of turk's solution in wbc counting?
to stain it.
What is used to sort DNA into different lenghts?
Gel Electrophoresis
What is the purpose of a gel electrophoresis?
to separate a mixture usually that of DNA so that it can be found to correspond with that found at a crime scene
One lane of an electrophoresis gel shows a 100bp ladder. What is the purpose of this ladder?
It is used as a marker for molecular weight.
What is the purpose of the crystal violet in the gram stain procedure?
Its the primary stain of the procedure. IT stains the Gram positive organisms
What is the purpose of crystal violet in the Gram's stain procedure?
Its the primary stain of the procedure. IT stains the Gram positive organisms
What is the purpose of emulsifying the bacteria in the capsule stain?
to stain the outside and the inside of the cell.. so the capsule appears transparent
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
What makes DNA visible?
You can an electrophoresis gel and then stain the gel using a solution such as coomassie blue to make the bands visible. Alternatively, you can stain a cell containing DNA by using acridine orange. It is necessary to observe these under an electron light microscope.
For what purpose might one use capillary electrophoresis?
Capillary electrophoresis is a technique used in laboratories to separate molecules based on their charge in order to study and analyze them. Capillary electrophoresis uses an electric charge to force the movement of molecules since each molecule will go a varying distance based on the weight of the molecule and their charge. Some areas of study that use capillary electrophoresis include DNA sequencing and pharmaceutical analysis.
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
