The enzyme DNA polymerase ( Taq polymerase) used in the PCR requires Mg 2+ ions for its functioning.These Ions act as cofactors for the enzyme . Hence the requirement for the use of Mg Cl2 in PCR reactions.
Asked in Science, Biotechnology
What is the purpose of the buffer in PCR?
The purpose of the buffer in PCR, I assume you talking about the 5 or 10 times PCR buffer that is provided with enzyme. Buffer is needed to give the correct pH and pottasium ion concentration for the DNA polymerase enzyme (usually DNA polymerase from Thermus aquaticus) to function. ...
Asked in Biology, Physical Therapy (physiotherapy)
Types of pcr?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR ...
Asked in Biology, Biotechnology
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on. ...
Asked in Microbiology, Genetics
What is the difference between real time pcr and conventional pcr?
Real time PCR is quantitative while conventional PCR is qualitative. We cannot quantitate the product in case of normal PCR which is possible with real time PCR. megha ...
Asked in Biology
Why is PCR used?
PCR is a method to easily amplify your gene copies. It is largely used before cloning and sequencing of gene of interest. This revolutionized the field of molecular biology. There are different PCR methods developed such as multiplex, nested, Q PCR, RT PCR and so on. ...
How many atoms are in the element MgCl?
If unbalanced the molecule MgCl will have one atom of Mg and one of Cl and therefore two atoms. in real life you would not have MgCl, you would have MgCl2 (where 2 is a subscript). This means there is one atom of Mg and two of Cl and therefore 3 atoms in total. ...