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The resultant crystal violet- iodine (CV-1) complex serves to intensify the-color of the stain. At this point, all cells will appear purple black.
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What is the purpose of mordant stain in a differential staining procedure?
The resultant crystal violet- iodine (CV-1) complex serves to intensify the-color of the stain. At this point, all cells will appear purple black.
What is the purpose of differential staining?
differential staining is a staining technique used to stain colorless bacteria against a dark background.
What is the purpose of the heat during the staining procedures?
It acts as the mordant to soften the mycolic acid so that the stain can penetrate the cell.
What is the purpose of the heat during the acid fast staining procedure?
The heat is used to drive the primary stain, carbol fuchsin, into the waxy cell wall of acid-fast bacteria. This allows the stain to penetrate the mycolic acid in the cell wall, making the bacteria resistant to decolorization with acid-alcohol.
What is the principal of flagellar staining?
flagella stain Purpose: To determine the presence/absence and location of flagella on various microorganisms Principle: Because bacterial flagella are very thin and fragile a special stain (flagella stain) is prepared that contains a mordant. This mordant allows piling of the stain on the flagella, increasing the thickness until they become visible. Various arrangements of flagella are seen on different cells.
What is the purpose of spore staining?
not sure
What is mordant used in the gram staining procedure and its function?
A mordant is an element which aids the chemical reaction that takes place between the dye and the fibre so that the dye is absorbed. It may also mean incisive or insistent especially in questioning something.
What is the purpose of staining?
to detect bacterial stracture
What is the purpose of albert staining?
to detect bacterial stracture
Cite the purpose of following reagents in a differential staining procedure?
To distinguish between acid fast positive and acid fast negative bacteria. Acid fast positive bacteria will stain red to pink color and acid fast negative bacteria will stain blue. Acid fast positive bacteria have mycolic acid in their cell wall, which will stain with carbol fuchsin and not decolorize with acid alcohol. Acid fast negative bacteria do not have mycolic acid in their cell wall, and become decolorize with the acid alcohol. Counterstain of methylene blue needs to be done in order to see the acid fast negative.
What is thee purpose of adding methylene blue to the specimen?
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
What is the purpose of emulsifying the sample serum in staining procedure?
To promote the cells adhering to the glass slide since they can't be heat fixed. When preparing a capsule stain you have to aseptically add organisms and emulsify with a loop.
