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BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. This protein does not affect other enzymes that do not need it for stabilization.

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Q: What is the role of BSA in restriction enzyme activity?
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Probably the plasmid is partially uncutted and it is present in his coiled and supercoiled forms. you can add another control as the undigested construct. In addition you can add much more enzyme, change batch of EcoRI and digest overnight with BSA to exclude star activity.


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