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The plasmid sample is supposed to contain only the DNA fragmentWhy are there more than one band?
Wiki User
∙ 10y ago
Probably the plasmid is partially uncutted and it is present in his coiled and supercoiled forms. you can add another control as the undigested construct. In addition you can add much more enzyme, change batch of EcoRI and digest overnight with BSA to exclude star activity.
Wiki User
∙ 10y ago
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How many Ag atoms are there in a 88.1 gram sample of Ag?
A 88,1 gram sample of Ag contain 4,9185.10e23 atoms.
How many cytosine bases does a sample of DNA contain?
That depends on the particular sample of DNA.
How many moles of carbon monoxide are in 350 g sample?
350 g sample of CO contain 12,49 moles.
How does salmonella E. work in recombinant DNA?
E. Coli is typically used as the host of recombinant DNA cloning. The process is as follows (simple version): DNA sample is cut use restriction sites, and a primer is loaded The cut DNA is place in a vector, example is a plasmid The plasmid is inserted into the host, example is E.Coli The E.Coli produces the DNA
How many gold atoms does Au contain?
This depends on the mass of the gold sample.
Why is it that dried food sample can contain lower numbers of microorganisms?
Why is it that dried food sample can contain lower numbers of microorganisms
To perform Uranium-235 dating on a sample what must be true?
The sample must contain Uranium-235.
What must be true for radioactive dating to be possible with a certain sample?
The sample must contain radioactive elements.
If your nanodrop is giving you a plasmid DNA concentration of 178.9 ng/µL what is the volume of sample, you need to take in order to have 10 ng DNA final concentration?
This will give you info
How much Pu 239 is typically found a uranium sample?
A pure and fresh prepared uranium sample don't contain plutonium; only the irradiated (in a nuclear reactor) uranium contain plutonium.
How many Ag atoms are there in a 88.1 gram sample of Ag?
A 88,1 gram sample of Ag contain 4,9185.10e23 atoms.
How many cytosine bases does a sample of DNA contain?
That depends on the particular sample of DNA.
How many moles of carbon monoxide are in 350 g sample?
350 g sample of CO contain 12,49 moles.
How many grams does a 0.0233 kg sample contain?
23.3 grams
Which samples contain visible particles?
which sample contains visible particles
What are the number of sulfur atoms in a sample of 21.6 g of mercury1 nitrate?
Mercury I nitrate does not contain sulfur unless the sample is contaminated.
How does salmonella E. work in recombinant DNA?
E. Coli is typically used as the host of recombinant DNA cloning. The process is as follows (simple version): DNA sample is cut use restriction sites, and a primer is loaded The cut DNA is place in a vector, example is a plasmid The plasmid is inserted into the host, example is E.Coli The E.Coli produces the DNA
