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Why dmem can not be autoclaved?
DMEM (Dulbecco's Modified Eagle Medium) contains heat-sensitive components such as amino acids and vitamins that can be denatured or degraded by autoclaving, which involves high heat and pressure. Autoclaving can lead to changes in the composition of the medium, affecting cell growth and viability. Therefore, DMEM is typically sterilized by filtration using a sterile filter instead.
What is a cell culture how can A cell culture be useful to biologist?
A cell culture is where cells are grown under controlled conditions.
What is the role of DMSO in cell culture?
DMSO (dimethyl sulfoxide) is commonly used in cell culture as a cryoprotectant to prevent ice crystal formation and cell damage during freezing and thawing of cells. It helps preserve cell viability and functionality when cells are stored at low temperatures. DMSO is also used as a solvent for some compounds and reagents in cell culture experiments.
What is the role of fixative in lymphocyte culture?
Fixative in lymphocyte culture helps to preserve the morphology of the cells by preventing them from moving or changing shape during processing and staining. It also helps to prevent cell loss or detachment from the culture dish. Fixatives such as formaldehyde also help to cross-link cell proteins, allowing for better visualization under the microscope.
What is tissue culture treated cell culture plate?
A tissue culture treated cell culture plate is a specialized plastic plate that has been treated to promote the adherence and growth of cells, particularly for use in cell culture experiments. The treatment usually involves coating the surface with materials such as collagen or gelatin to enhance cell attachment and growth, providing a more suitable environment for cell culture. This type of plate is commonly used in research laboratories for growing cells in vitro.
Growth media for streptomycin?
streptomycin is an antibiotic used in different types of cell culture media. Generally, this antbiotic is incorporated into DMEM (Delbucose Minimum Essential Medium) and used to culture mammalian cells
Why dmem can not be autoclaved?
DMEM (Dulbecco's Modified Eagle Medium) contains heat-sensitive components such as amino acids and vitamins that can be denatured or degraded by autoclaving, which involves high heat and pressure. Autoclaving can lead to changes in the composition of the medium, affecting cell growth and viability. Therefore, DMEM is typically sterilized by filtration using a sterile filter instead.
What is a cell culture how can A cell culture be useful to biologist?
A cell culture is where cells are grown under controlled conditions.
What is a cell culture how can cell culture be useful to biologists?
A cell culture is where cells are grown under controlled conditions.
What are the characteristics and functions of a confluent cell in cell culture?
Confluent cells in cell culture refer to cells that have grown to cover the entire surface of the culture vessel, forming a single layer. These cells are in contact with each other and exhibit characteristics such as tight junctions and a uniform appearance. Confluent cells play a crucial role in maintaining cell viability, promoting cell-to-cell communication, and supporting various cellular functions, such as differentiation and proliferation.
What is the role of DMSO in cell culture?
DMSO (dimethyl sulfoxide) is commonly used in cell culture as a cryoprotectant to prevent ice crystal formation and cell damage during freezing and thawing of cells. It helps preserve cell viability and functionality when cells are stored at low temperatures. DMSO is also used as a solvent for some compounds and reagents in cell culture experiments.
What is the role of FBS in mammalian cell culture?
FBS (fetal bovine serum) is commonly used in mammalian cell culture as a source of essential nutrients, growth factors, and hormones to support cell growth and proliferation. It provides a suitable environment for cells to thrive and maintain their physiological functions in vitro.
What role does sodium bicarbonate in cell culture?
It works to maintain the pH of the media in an acceptable range by acting as a buffer. see related link
How is fetal bovine serum made and what is its role in scientific research and cell culture applications?
Fetal bovine serum is derived from the blood of cow fetuses. It contains essential nutrients and growth factors that support the growth of cells in scientific research and cell culture applications. Its role is to provide a suitable environment for cells to grow and thrive outside of the body.
What do you call a group of cells grown in a nutrient solution from a single cell?
Cell culture is a nearly universally used technique to grow material for biological testing. In cell culture, an original population of seed cells divide under tightly controlled conditions to propagate the culture. All of the daughter cells from the original population are clones.
How does the presence of dmem glucose affect cellular metabolism in a laboratory setting?
In a laboratory setting, the presence of dmem glucose can affect cellular metabolism by providing a source of energy for the cells to use in various metabolic processes. Glucose is a key fuel for cells, and its presence can influence the rate at which cells grow, divide, and produce energy.
What is the role of folk dances in culture of the people?
what is the role of folk dance in culture of the people
