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Q: What is the role of crystal violet in biofilm assay?
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What important role does ozone play in protecting the earth?

It protects us from harmful ultra violet radiation


What effect does the age of a culture have on its gram stain?

Yes, the age of the culture may influence the results of the stain. With the current theory behind gram staining, it is thought that in gram-positive bacteria, the crystal violet and iodine combine to form a larger molecule that precipitates out within the cell. The alcohol/acetone mixture then causes dehydration of the multilayered peptidoglycan in the gram-positive call wall, thus decreasing the space between the molecules and causing the cell wall to trap the crystal violet-iodine complex within the cell. In the case of gram-negative bacteria, the alcohol/acetone mixture, being a lipid solvent, dissolves the outer membrane of the gram-negative cell wall (and may also damage the cytoplasmic membrane to which the peptidoglycan is attached). The thin layer of peptidoglycan is unable to retain the crystal violet-iodine complex and the cell is decolorized. It is important to note that gram-positivity (the ability to retain the purple crystal violet-iodine complex) is not an all-or-nothing phenomenon but a matter of degree. There are several factors that could result in a gram-positive organism staining gram-negatively: 1. The method and techniques used. Overheating during heat fixation, over decolorization with alcohol, and even too much washing with water between steps may result in gram-positive bacteria losing the crystal violet-iodine complex. 2. The age of the culture. Cultures more than 24 hours old may lose their ability to retain the crystal violet-iodine complex. 3. The organism itself. Some gram-positive bacteria are more able to retain the crystal violet-iodine complex than others.


What is an autolysin?

An autolysin is an enzyme produced by bacteria that can break down their own cell walls. It is involved in processes like cell division and the release of DNA during bacterial cell death. Autolysins play a role in biofilm formation and the spread of antibiotic resistance genes.


Are infrared rays absorbed by the ozone layer and do they protect earth?

The ozone layer performs a unique role in absorbing UV-B from our Sun, a role no other common gas performs. Additionally, ozone absorbs uniquely a band of far infrared radiation, that otherwise has absolutely no harmful effects on life on Earth. Infrared presents no risk to life, but can serve to keep Earth warm. yes or no?


Will different liquids effect the growth of a plant?

Crystals are grown for both scientific purposes and for experimentation in labs or science fairs, to demonstrate the various properties of different kinds of crystal formation. In all kinds of formation, the constant temperature plays an important role in crystal development, affecting not only how fast the crystals develop but the ways in which they grow. Read more: How Does Temp Affect the Growth Rate of Crystals? | eHow.com http://www.ehow.com/about_6318908_temp-affect-growth-rate-crystals_.html#ixzz2K4J5sgBq

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If the mitochondrion is fundmental to the mtt assay which organelle plays a key role in the neutral red viability assay and why?

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act as stabilizer.


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It is used to suspend the cells prior to lysis.


What is the real name of the girl who played violet baudelaire?

The actress who plays the role of Violet Baudelaire is Emily Browning.


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The flower in The Westing Game is a chrysanthemum. It plays a significant role in the story and is associated with one of the characters.


What is the role of main character in midnight bu Jacqueline Wilson?

The main character is Violet


What actors and actresses appeared in Billy in Armor - 1913?

The cast of Billy in Armor - 1913 includes: Violet Horner as Violet Billy Quirk as Billy Bud Ross as Secondary Role


On the TV series Wings who plays the role of Helen Chappel?

Crystal bernard


What important role does ozone play in protecting the earth?

It protects us from harmful ultra violet radiation


Who declined a role in Toy Story and then took one in Monsters Inc?

Billy Crystal.


Who is violet westing?

Violet Westing is a fictional character from the book "The Westing Game" by Ellen Raskin. She is the daughter of Samuel Westing, the eccentric millionaire whose death sets off the mystery at the center of the story. Violet is portrayed as a kind and caring woman who ends up playing a key role in unraveling the secrets of the Westing estate.