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Q: What is the role of gelatin in bromelain enzyme assay?
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Why is fresh pineapple not used in gelatin desserts?

Pineapples contain an enzyme that is used to aid digestion and to tenderize meat, which will not only be tender but will likely fall apart. This is the reason that fresh pineapple cannot be used in gelatin desserts as it will break down the protein in the gelatin. Since heating destroys this enzyme, gelatin desserts are successfully made using canned pineapple. Bromelain is a proteolytic (protein-dissolving) enzyme similar to papain in unripe papaya and ficin in fresh figs. It can be so strong that plantation and cannery workers must wear rubber gloves to prevent their hands from being eaten away. The enzyme has also proven to break down many harmful proteins and may well play an important role in thrombosis treatments in the future. Thrombosis is a blockage of blood vessels by clots and are responsible for a significant number of deaths each year. Heart attacks are most often the result of blockage in the blood vessels serving the heart, while strokes are the result of similar blockages in the vessels of the brain. The damage done from these clots is caused largely by a protein called fibrin, which bromelain is able to break down. Progress in this area is slow, however, as the medical establishment is still reluctant to admit that diet plays any significant part in illness or recovery. Bromelain is extracted mainly from the stems of pineapple, although it is also present in the fruit and leaves.


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