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Gene tagging facilitates systematic genomic and proteomic analyses but chromosomal tagging typically disrupts gene regulatory sequences. Here a seamless gene tagging approach was discovered that preserves endogenous gene regulation and is potentially applicable in any species with efficient DNA double-strand break repair by homologous recombination. In yeast a tag is typically introduced into the genome together with a marker gene used to select for positive transformants.

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11y ago
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12y ago

Genes governing traits of interest can be located on the chromosomes via linkage to mapped RFLP markers in a process known as "gene tagging" (Burr et al 1988).

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Q: What is the role of gene tagging in gene analysis?
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