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The pigment molecules and electron transport chains involved in the light-dependent reactions of photosynthesis are embedded in the thylakoid membrane. As energy is released from electrons traveling through the chain of acceptors, it is used to pump protons (that is, H+ ions) from the stroma of the chloroplast across the thylakoid membrane and into the center of the thylakoid. Thus, protons accumlate within the thylakoids, lowering the pH of the thylakoid interior and making it more acidic. A proton gradient possesses potential energy that can be used to form ATP.

Protons are prevented from diffusing out of the thylakoid because the thylakoid membrane is impermeable to protons except at certain points bridged by an enzyme called ATP synthase. This protein extends across the thylakoid membrane and forms a channel through which protons can leave the thylakoid. As the protons pass through ATP synthetase, energy is released, and this energy is tapped by ATP synthase to form ATP from ADP and inorganic phosphate. The coupling of ATP synthesis to a protein gradient formed by energy released during electron transport is called chemiosmosis.

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Trever Windler

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3y ago

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