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Q: What is the significance of nominal wavelength of a substance?
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The nominal bore of a pipe is its internal diameter. The nominal bore determines how much volume you have in the pipe to transport your particular substance.


In relation to spectroscopy what is the Optimum wavelength and how to find it?

The optimum wavelength is the wavelength by which the most light is absorbed by a substance. It can be found by finding the highest absorbance obtained when testing the substance's absorbance at various wavelengths. The wavelength that results in the greatest light absorbance is your optimum wavelength.


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Rf Values determine the solubility of a substance with respect to a certain solvent.


Why are particular wavelengths used for particular spectrophotometric assays?

A particular wavelength used for particular spectrophotometric assays is based upon the absorbance pecularity of the substance in consideration/used for the assay. That particular substance absorbs at a particular wavelength leaving other things alleviating the chances of background contamination/noise.


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Basically radius of gyration of a substance is defined as that distance from the axis of rotation from which if equivalent mass that of the substance is kept will have exactly the same moment of inertia about that axis of the substance.


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