0
Why are particular wavelengths used for particular spectrophotometric assays?
Wiki User
∙ 11y ago
A particular wavelength used for particular spectrophotometric assays is based upon the absorbance pecularity of the substance in consideration/used for the assay. That particular substance absorbs at a particular wavelength leaving other things alleviating the chances of background contamination/noise.
Wiki User
∙ 11y ago
Add your answer:
Earn +20 pts
Calibrated the spectrophotometer with?
Spectrophotometric standards are used - crystals or solutions.
What are the differences between AAS and AES in terms of the source used?
Emission spectroscopyis a spectroscopic technique which examines the wavelengths of photons emitted by atoms or molecules during their transition from an excited state to a lower energy state. Each element emits a characteristic set of discrete wavelengths according to its electronic structure, by observing these wavelengths the elemental composition of the sample can be determined. Absorption spectroscopy measures the loss of electromagnetic energy after it illuminates the sample under study. For example, if a light source with a broad band of wavelengths is directed at a vapour of atoms, ions, or molecules, the particles will absorb those wavelengths that can excite them from one quantum state to another.
What is a cronical flask used for?
to pour particular liquid into it, used mainly for science
How does immunodiffusion assay differ from immunoelectrophoresis assay?
an electric current is passed through the agar in electroimmunodiffusion assays and therefore separates antigens based on their isoelectric point. Immunodiffusion does not involve electrophoresis and can be used to assess Ab to Ag binding through the visualization of a precipitate line
What is non aqueous titration?
Nonaqueous titration is the titration of substances dissolved in nonaqueous solvents. It is the most common titrimetric procedure used in pharmacopoeial assays and serves a double purpose: it is suitable for the titration of very weak acids and very weak bases, and it provides a solvent.
What amino acid residues are detected when the spectrophotometric assay is used to quantify proteins?
In Lowry spectrophotometric assay, tryosine, tryptophan, cysteine, histidine, and asparagine.
Calibrated the spectrophotometer with?
Spectrophotometric standards are used - crystals or solutions.
What was luminol originally used for?
used by biologists in cellular assays for the detection of copper, iron, and cyanide.
Why is the wavelength 275 nm used to measure absorbance of caffeine?
Different substances have their characteristic behavior for different wavelengths; I assume caffeine easily absorbs that particular frequency.
How do AM radio and television wavelengths compare with X rays?
All wavelengths used for communication are longer than all infrared, visible, ultraviolet, and X-ray wavelengths.
What test is used for valley fever?
A number of tests are used for Valley fever: chest x-rays, biopsy, sputum exam, and complement fixation assays.
What measurement is used for the wavelengths of color?
Wavelengths of visible light are measured in nanometres. different colours are at different wavelengths. The range runs from violet at around 380nm to red at around 750nm.
What can a diffraction grating be used for?
measuring wavelengths of light
Why NaCl is not used in FTIR?
It is used but not after wavelengths higher than 15 micrometres.
Can heat be used as an energy source when there is no light for plant growth?
No. Certain wavelengths of light are needed for photosynthesis. The appropriate wavelengths are not present in purely thermal energy.
Are wavelengths used in photosynthesis primarily infrared and ultraviolet?
No, the wavelengths are between visible light(around 400-600nm i guess)with the exception of the wavelength of green light.
What is the purpose of colorimeter?
Chemists will use colorimetry to determine various states of chemicals in solution. The equipment has many uses.
