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The purple stained bacteria are called gram positive bacteria and retain the stain after washing by having a thick cell wall one one layer of a heavily crossed linked material called peptidoglycan. ( Google that ) The red stained bacteria are gram negative because they posses two thinner layers of peptidogylcan cell wall that that do not hold the gram stain and it is mostly washed away in rinsing the bacterial samples.
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∙ 13y ago
Wiki User
∙ 8y agoGram-positive bacteria stain purple, and gram-negative bacteria stain pink/red.
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What is the different reaction of Gram-negative and Gram-positive bacteria to Gram's stain testing?
Gram-positive bacteria which take up the stain turn purple, while Gram-negative bacteria which do not take up the stain turn red.
What is the difference between gram plus and?
Gram positive bacteria stain purple with the Gram stain. This is because of the fact that they contain a thick layer of murein in their cell wall, which takes up the stain very well. Gram negative bacteria, however, do not display the thick layer of peptidoglycan on their outer surface. Therefore, they stain red with the counter stain.
What is the conclusion of a gram stain of purple rods and cocci through a microscope?
This is a Gram Stain. If the technique was proper, the red rods are Gram-negative and the purple cocci are Gram-positive. This staining technique is used to help identify various bacteria. The Gram-positive bacteria that are purple hold the stain due to it's layered cell membrane. It contains a peptidoglycan layer that acts as a lattice trapping the crystal violet-Iodine dye complex.
Do gram-positive cells carry a net positive charge?
Gram-positive does not refer to a positive charge, but to the purple color of the stain. Gram-negative bacteria do not retain the purple dye and are instead red in color.
How does thickness of peptidoglycan affect gram stain outcome?
For Gram Stain experiment. The gram-positive, g+, which stained purple in color (use Crystal violet) as the bacteria contain a thicker layer of peptidoglycan with no lipopolysaccharide and protein bound the outer membrane of the bacteria (bacteria A). The gram-negative, g-, which stained in red (if the safranin (red dye/counter stain is use) appear due to the bacteria (bacteria B) which lack of peptidoglycan and it is bound by a lipopolysaccharide and protein layer. The thicker the peptidoglycan, the more coloring agent (crystal violet ions, CV+) it can absorb. Hence, give more purple in color to the bacteria and this will be the gram-positive, g+ bacteria. On the other hands, bacteria B seem has thinner peptidoglycan thus it absorb less purple color from the CV+, and after decolourise (using alcohol) and stained with safranin (red dye), only the bacteria with lack of peptidoglycan change to red hence we can say bacteria B as gram-negative, g-. Hence the outcome is the bacteria with thicker peptidoglycan layer be the gram-positive, g+(purple color) while the thinner peptidoglycan layer be the gram-negative, g-. (red color)
What will happen after treating different types of bacteria with a Gram stain?
Some bacteria will appear purple, whereas other bacteria will appear red.
What color is gram-negative stain?
Red or pink. Gram Positive = Purple or Violet, Gram Negative = Red or Pink. I just think of the mnemonic Positive-Purple both starting with P.
What is the appearance of gram negative bacteria after addition of mordant in the gram stain?
Red/pink
What is there in the structure of bacteria that cause some to stain purple and other to stain red?
The purple stained bacteria are called gram positive bacteria and retain the stain after washing by having a thick cell wall one one layer of a heavily crossed linked material called peptidoglycan. ( Google that ) The red stained bacteria are gram negative because they posses two thinner layers of peptidogylcan cell wall that that do not hold the gram stain and it is mostly washed away in rinsing the bacterial samples.
What is the different reaction of Gram-negative and Gram-positive bacteria to Gram's stain testing?
Gram-positive bacteria which take up the stain turn purple, while Gram-negative bacteria which do not take up the stain turn red.
Are there any circumstances where Gram positive bacteria stain red and not purple?
Lol. Uni Adelaide HumPer
What does differentating mean?
It means to see the differences between things. For example some bacteria will stain red and others will stain blue/purple, this differences between them helps us tell them apart.
What differention mean?
It means to see the differences between things. For example some bacteria will stain red and others will stain blue/purple, this differences between them helps us tell them apart.
What is the difference between gram plus and?
Gram positive bacteria stain purple with the Gram stain. This is because of the fact that they contain a thick layer of murein in their cell wall, which takes up the stain very well. Gram negative bacteria, however, do not display the thick layer of peptidoglycan on their outer surface. Therefore, they stain red with the counter stain.
What is the conclusion of a gram stain of purple rods and cocci through a microscope?
This is a Gram Stain. If the technique was proper, the red rods are Gram-negative and the purple cocci are Gram-positive. This staining technique is used to help identify various bacteria. The Gram-positive bacteria that are purple hold the stain due to it's layered cell membrane. It contains a peptidoglycan layer that acts as a lattice trapping the crystal violet-Iodine dye complex.
What is the advantage of the gram stain over the simple stain?
gram staining differentiates between gram negative and gram positive bacteria by showing different colors. it shows blue or purple like color for gram positive bacteria and red color for gram negative bacteria. where as simple stain gives the same color to all the types of bacteria. hence it is difficult to differentiate between them.
Explain the mechanism of gram staining?
The slide is colored with purple and red stains, then examined under a microscope. The color of stain picked up and retained by the bacteria (purple or red), their shape (such as round or rectangle), and their size provide valuable clues to their identity
