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When you added methylene blue to the living yeast cell, the methylene blue is actually a dye so you could see the cells, therefore, the yeast cell became blue. It is much the same with hair dye. If you have a bottle of blue hair dye, it will dye your hair blue.
Polluted water sample will decolorize the methylene blue solution faster than a pure water sample.
Methylene blue is used as an indicator for the presence of certain Ions such as the presence of HS - ion. spectrophotometry is used once the methylene blue has reacted to determine the quantity of said ion based on the intensity of blue that the reacion produces. Using a spectrophotometer you can determine the intensity and compare it with a set scale that has been calibrated with known concentrations of this ion.
One substance that has a similar function as methylene blue is crystal violet. It is commonly used in staining techniques for microbiological studies and exhibits similar properties in terms of staining cells and tissues.
NADH
If methylene blue is blue, it means that the compound is in its oxidized state (methylene blue) and has accepted electrons. Methylene blue can exist in both oxidized (blue) and reduced (colorless) forms depending on its redox state.
i think the methylene blue will be make aqua blue because the charcoal will penerate the color of methylene blue,,,there are absorption process,,,in the charoal between the methylene blue.... (kharlz)
Methylene blue stains everything blue.
Polychrome methylene blue is commonly used in histology to stain acidic polysaccharides, mucins, and cartilage sections. It helps differentiate structures and aids in the visualization of specific components within tissues under a microscope.
Methylene blue can act on only dead yeast cells because it is able to penetrate the cell membrane easier when the cell is dead, allowing it to enter and stain the cell. In live yeast cells, the cell membrane is intact and acts as a barrier, preventing methylene blue from entering and staining the cell.