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Q: What is the uncontrolled variable in paper chromatography of pigments?
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What procedure did you to separate plant pigments?

Paper chromatography can be used to separate pigments in plants. The pigments are separated into chlorophylls (greenish colors) and carotenoids (yellowish colors).


What is the purpose of the solvent in chromatography?

The basic purpose of the solvent is to move the pigments up the chromatography paper through capillary action so that the pigments can be separated. The basic importance of chromatography is to separate different solvents by their molar mass. In doing so, one can separate the different pigments within a solution and measure how much of each pigment is present.


Why does carotene travel farthest in chromatography of leaf pigments?

it has more affinity (attraction) to the solvent than to the paper


What procedure did you use to separate plant pigments?

Place leaf in beaker and heat with Bunsen burner until water has discolored and boiled, then place leaf in test tube filled methylated spirits and place in same beaker as before with the burner turned OFF. Methylated spirits boils at around 80 degrees Celsius so when placed in heated water it will boil. This process will separate the chlorophyll (pigment) in the leaf.


How do you separate ink in colour?

you put ink on the bottom of the chromatography paper and dip one end of of it into water and leave it to stoke up the paper, separating the ink pigments


What are the variables in a plan and design paper chromatography lab?

responding variable-distance of components( Rf values)


Uses for paper chromatography?

They are used in many scientific studies to identify unknown organic and inorganic compounds. They are also used in crime scene investigation, DNA and RNA sequencing, among others. Essentially, any solution can be separated through some form of chromatography.


What is the interpretation of paper chromatography?

whate is interpretation in paper chromatography


Disadvantages of paper chromatography?

The only disadvantage, like any paper chromatography method, is that the test take a lot of time - typically 45 to 90 minutes.This method also yield little amount of pigments when it comes to the extraction of the isolated pigments. Several tests must be run to gather a respectable amount for further chemical tests.


What property of the ink was used to separate the colours?

The process is called chromatography. The property is the attraction of pigments to a liquid or solution (such as water) and its ability to dissolve in the solution. In paper chromatography, a pigment that dissolves easily in water will travel farther up the paper as opposed to an oil, that does not dissolve in water.


What can you use instead of chromatography paper in a paper chromatography test?

newspaper


How is paper chromatography used in everyday life?

it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.