0
Want this question answered?
Be notified when an answer is posted
Add your answer:
Earn +20 pts
What is the role of DEPC in RNA isolation?
It inactivate the RNase and prevent RNA to denature.
When edta is insoluble in distilled water then how to make 0.01N edta solution?
use heat to heat the solution and add EDTA slowly to dissolve it.
Role of extraction buffer in DNA isolation?
TE buffer contains EDTA, which is a strong chelating agent. It chelates the Mg2+ ions present in the solution. Since endonucleases use Mg2+ for their activity, degradation is slowed or checked using this buffer. This buffer is also maintained at a pH of 8.0 for the same reason. At this pH, the endonucleases show least activity. All in all, the DNA or RNA sample that we have is safe from getting degraded.
Determination of magnesium in tap water?
u can use titration with EDTA or use flame atomic absorption.. but titration with EDTA is the easiest
Role of EDTA in DNA isolation?
it is chealeting agent and has great affinity with metal ions and mg- ions present in dnase as a cofactor and responsible for dnase action that degreded DNA hear edta bide with mg- ions and stop the action of dnase.
Tris EDTA buffer in plant DNA isolation?
Chelating agent
Role of te in dna extraction?
TE stands for Tris and EDTA. The Tris buffers the water to prevent acid hydrolysis of the DNA/RNA. The EDTA chelates divalent cations that can assist in the degradation of RNA.
What is the role of DEPC in RNA isolation?
It inactivate the RNase and prevent RNA to denature.
What is the role of NaCl in RNA isolation?
The role of NaCl or sodium chloride in RNA isolation is part of the denaturing process. It is often called the wash step.
What is the role of TRIreagent in RNA isolation?
Break open the cells, stabilize RNA, inhibit RNAse.
Why ph8 is maintained during isolation of plasmid?
EDTA is dissolved only at pH8. EDTA serves as an important chelating agent to kill the contaminating DNAses. Also this is close to the physoological pH which is pH7.
How is RNA removed from DNA during DNA isolation?
Most often, RNA is removed using the enzyme RNAase
What is role of BCP bromo chloropropane in RNA isolation?
for phase separation
Role of chloroform in RNA isolation?
it solubilize the lipids and protein and remove them.
What is the use of guanidine isothiocyanate and NaOAc in the isolation of RNA?
Guanidine isothiocyanate helps denature proteins from the RNA to allow them to be separated from protein for the best isolation of nucleic acids from proteins (can collect all 3 if using TRIzol like reagents)NAoAc (sodium acetate) usually in 3M/pH8 is used later in the steps for nucleic acid isolation as the salt for ethanol precipitation. If you are going to be doing RNA transcription off of DNA templates that you are precipitating, it is best to use Nh4oAC (ammonium acetate) as the ion is nicer to RNA polymerases once templates are cleaned and being transcribed.
What is the role of 1 percent of NaOH in the isolation of RNA?
it helps in precipition step...
What is the function of MOPS buffer RNA isolation?
It act as a buffer in Northern blotting.
