Magnesium and EDTA react very quickly through a highly energized ionization reaction in which the EDTA can lose up to 4 hydrogens. The powerful ionization drives the reaction to completion. You can titrate the magnesium and calculate the point of reaction to better understand the mechanism
A complexation reaction where EDTA surrounds the metal ion encapsulating it.
complexation or chelation reaction
Ca-EDTA is more stable.. its stability constant is above 10 wheras Mg's is below 9, as to why this is I dont know
EDTA removes the ions that lactase needs to function as an enzyme. If enough EDTA is added, lactase will no longer have any of it's ion cofactors to aid in the break down of lactose.
To accurately calculate the molarity of an EDTA solution you need to determine the number of moles present and liters of solution. Take these two totals and divide them to determine molarity.
it is organic
complexation or chelation reaction
It Inhibits the PCR reaction by chelating the magnesium ions.
magnesium
EDTA is ethylenediaminetetraacetic acid; the chemical formula is C10H16N2O8.EDTA can react with calcium and magnesium ions forming insoluble precipitates.
u can use titration with EDTA or use flame atomic absorption.. but titration with EDTA is the easiest
The conditional constant= 1.8*1010
Ca-EDTA is more stable.. its stability constant is above 10 wheras Mg's is below 9, as to why this is I dont know
A useful indicator for calcium titrations with EDTA in the presence of magnesium.
if edta is fresh reaction will preceed faster other wise it will take time
EDTA removes the ions that lactase needs to function as an enzyme. If enough EDTA is added, lactase will no longer have any of it's ion cofactors to aid in the break down of lactose.
to make the reaction and associated calculations more complicated
Replacement Titration: In this method the metal, which is to be analyzed, displaces quantitatively the metal from the complex. When direct or back titrations do not give sharp end points, the metal may be determined by the displacement of an equivalent amount of Mg or Zn from a less stable EDTA complex. Mn+2 + Mg EDTA---> 2 Mg+2 + Mn EDTA-2 Mn displaces Mg from Mn EDTA solution. The freed Mg metal is then directly titrated with a standard EDTA solution. In this method, excess quantity of Mg EDTA chelate is added to Mn solution. Mn quantitatively displaces Mg from Mg EDTA chelate. This displacement takes place because Mn forms a more stable complex with EDTA. By this method Ca, Pb, Hg may be determined using Eriochrome blackT indicator.