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Electroporation is the technology that is connected to the increase of electrical conductivity that is seen in the cell. This can cause the cell membrane to weaken and be more easily punctured.

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How electroporation increase permeability of plasma membrane?

Electroporation is a dynamic phenomenon that depends on the local transmembrane voltage at each point on the cell membrane. It is generally accepted that for a given pulse duration and shape, a specific transmembrane voltage threshold exists for the manifestation of the electroporation phenomenon (from 0.5 V to 1 V). This leads to the definition of an electric field magnitude threshold for electroporation (Eth). That is, only the cells within areas where E≧Eth are electroporated. If a second threshold (Eir) is reached or surpassed, electroporation will compromise the viability of the cells, i.e., irreversible electroporation.[2]


What are the differences between heat shock and electroporation methods for transforming cells?

Heat shock and electroporation are two methods used to transform cells by introducing foreign DNA into them. Heat shock involves briefly exposing cells to high temperatures, which increases their permeability and allows the foreign DNA to enter. Electroporation, on the other hand, uses an electric field to create temporary pores in the cell membrane, through which the foreign DNA can pass. In summary, the main difference between heat shock and electroporation methods is the mechanism by which they make cells more receptive to foreign DNA.


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What are the differences between electroporation and heat shock methods for introducing foreign DNA into cells?

Electroporation and heat shock are two methods used to introduce foreign DNA into cells. Electroporation involves applying an electric field to create temporary pores in the cell membrane, allowing the DNA to enter the cell. Heat shock, on the other hand, involves briefly exposing the cells to high temperatures, which causes the cell membrane to become more permeable, allowing the DNA to enter. In summary, electroporation uses an electric field to create pores in the cell membrane, while heat shock uses high temperatures to make the membrane more permeable.


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