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Well it depends on which type of agar preparation you use. For example, if you use a mannitol NaCl salt agar it'll contain a red phenol dye which is a pH indicator. If a microbe is pathogenic it'll ferment the mannitol into acids which turn the red indicator yellow. Non-pathogenic bacteria such as S. epidermidis will not ferment the mannitol and so no colour change will result.
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
Mannitol salt agar is selective for gram positive bacteria, and differential for mannitol fermenters. Phenol red is the indicator containing the enzyme mannitol.
Mannitol Salt Agar Complex is a synthetic medium because it is prepared from pure chemical substances and the exact composition of the medium is known.
Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.
Well it depends on which type of agar preparation you use. For example, if you use a mannitol NaCl salt agar it'll contain a red phenol dye which is a pH indicator. If a microbe is pathogenic it'll ferment the mannitol into acids which turn the red indicator yellow. Non-pathogenic bacteria such as S. epidermidis will not ferment the mannitol and so no colour change will result.
In my result i isolate bacillus sp on mannitol salt agar but i expect that this media has deoration or expaired
Mannitol salt agar is selective for gram positive bacteria, and differential for mannitol fermenters. Phenol red is the indicator containing the enzyme mannitol.
Mannitol salt agar inoculated with Micrococcus luteusshowing no fermentation of mannitol (pink medium). The colonies show a yellow pigment which is characteristic of M. luteus.
Mannitol salt agar is one of the best agar fro isolation of Staphylococcus aureus
Mannitol Salt Agar Complex is a synthetic medium because it is prepared from pure chemical substances and the exact composition of the medium is known.
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Gram-positive bacteria grows in Mannitol agar because it contains a high level of salt. This type of agar allows only certain types of bacteria to grow, making it selective.Ê
No, K. pneumoniae will not grow on Mannitol Salt Agar- gram negative organisms cannot grow on MSA, and K. pneumoniae is gram negative.
7.5% NaCl
No it doesn't because it doesn't ferment mannitol. P. mirabilis can actually grow on a MAC agar, but it is colorless. I actually had this lab in Micro260 today.An MSA agar has 7% high salt concentration and provides growth for S. aureus bacteria turning it yellow/gold. An MSA agar is selective for osmotolerant bacteria meaning for bacteria that can grow in different salt concentrations, and it's differential because it can ferment mannitol.
mannitol is a type of sugar, so it supplies the carbon in the MSA medium