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The darkfield microscope. "darkfield microscopy"
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∙ 14y ago
Wiki User
∙ 9y agoThe darkfield microscopic technique gives quite similar a field in appearance to that seen in the negative stain. A negative stain leaves the sample bright against a dark background.
Wiki User
∙ 11y agoDark Field Microscopy.The organism appear bright against a dark background.
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∙ 14y agoWhat microscopic technique gives a field similar in appearance to that seen in a negative stain?
Wiki User
∙ 14y agoDark field microscopy. Both produce a dark background with lighter subjects.
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∙ 13y agoDarkfield microscopy
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∙ 13y agodark field
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What is the definition of the term fluorescence microscopy?
The term "fluorescence microscopy" is a type of light microscopy in which the specimen is irradiated at wavelengths that excite fluorochromes. In medicine, it is used to detect antigens.
Which microscope would be MOST useful for examining the contours of the surface of a bacteria cell?
It depends on the type of biofilm, what surface the biofilm is on, and what information you want to get by looking at the biofilm under a microscope. If you just want to look at how much of a surface is covered by a biofilm, you can use normal light trasmission microscopy (as long as the surface is transparent e.g. glass). Alternatively you could use epifluorescent microscopy in combination with a fluorescent stain. If you want to look at the structure of the biofilm, confocal laser scanning microscopy is probably the best as you can get a 3D image. Other useful types of microscopy include phase contrast and DIC, which allow you to look at the biofilm without staining it first.
What type of microscopy does not use light?
Electron microscopy; Scanning Electron Microscopes (SEM) and Transmission Electron Microscopes (TEM). The vacuum required for electron microscopy to work correctly precludes the observation of living organisms. Biological samples must be dried then coated with a conductive metal.
Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
What can an individual who is blood type AB negative do?
An individual who is blood type AB negative can receive any blood type that is negative. Somebody who is O negative can donate blood to anyone.
Which type of microscopy produces an image of unstained cells that is most similar to the one one achieved by negative staining?
Darkfield microscopy
How do you know that it is a negative or a positive bacteria?
By which type of stain colors it.
Which type of microscopy is used for colorless specimens?
Transmission electron microscopy
Which type of microscopy is useful for viewing colorless specimens?
Transmission electron microscopy
Does pledge polish stain kitchen cabinets?
It does not stain `cabinets if they are made entirely of wood. It will stain formica or similar type surfaces on cabinets. Also do not polish any metal with the Pledge.
Is there a gram negative streptococcus?
Yes, gram negative streptococcus does exists. This is a type of bacteria that cannot retain a violet stain after the decolonization step of testing.
What is the definition of the term fluorescence microscopy?
The term "fluorescence microscopy" is a type of light microscopy in which the specimen is irradiated at wavelengths that excite fluorochromes. In medicine, it is used to detect antigens.
What are the different terms associated with the microscopy?
Examples: type of ilumination, magnitude, type (optical, electron microscopy, scanning microscopy, etc.), working temperature (ambient or high temperature), resolution, etc. See also the link below.
What type of organism would you look for in a Gram stain of the blood or cerebrospinal fluid in a menin gococcal case?
A gram-negative diplococcus
Can you tell an organisms gram stain by simple staining?
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
What are the advantages of the simple stain technique over the negative stain technique?
Negative staining has a dark contrasted background and the bacteria is white. Simple staining has a white background and bacteria is the color depended on your stain color.Negative staining when prepared is NOT heat fixed but simple staining when prepared is heat fixed. Heat fixed means when preparing slide with bacteria on it, it is passed over some type of flame, like a Bunsen burner flame, three times or four times.
Chemical used to make a specimen visible?
A general term for a chemical that makes a specimen visible is a stain. There are many types of stains available, depending upon the structure you want to visualize and the type of microscope you want to use, e.g. fluorescent stains like DAPI for fluorescence microscopy, or hematoxylin and eosin (H and E) staining for brightfield microscopy.Immersion oil
