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restriction endonuclease
Rhett Strosin
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Why restriction enzyme is also called endonuclease enzyme?
Because these enzymes cut the DNA molecule at a particular site. But like scissors these are useful tools in genetic engineering or recombinant DNA technology.
Which tool do biologist use to cut a section of DNA from a chromosome?
Restriction enzymes
What is the molecule used to cut the gene from the DNA in an organism?
Restriction endonuclease
When is DNA cut during electrophoresis?
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
When scientist copy DNA they first have to cut out the part they want to copy . What molecule do they use to do this?
Restriction endonuclease.
When scientist copy DNA the first have to cut out the part they want to copy what molecule do they use to do this?
Restriction endonuclease.
When scientists copy DNA they first have to cut out the part they want to copy . what molecule do they use to do this?
Restriction endonuclease
When scientist copy DNA they first have to cut out the part they want to copy what molecule do they use to do this?
Restriction endonuclease.
When scientist copy DNA what do they have to cut out first?
Restriction endonuclease
What is the role of DNA helicase in the replication of DNA?
(Apex) It breaks apart the bases.
Where do they cut the DNA strand?
They direct a specific Restriction Enzyme to cut the Dna Exactly where required.
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmis into a bacterium?
I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes III. Extract plasmid DNA from bacterial cells IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA
