restriction endonuclease
The molecule used to find and cut DNA in genetic engineering processes is called a restriction enzyme.
Because these enzymes cut the DNA molecule at a particular site. But like scissors these are useful tools in genetic engineering or recombinant DNA technology.
Restriction enzymes
Restriction endonuclease
Electrophoresis technique is not designed to cut DNA molecule. When DNA is analyzed by electrophoresis to determine its molecular mass, the molecular biology engineer usualy digests the DNA molecule, before the electrophoresis, with specific enzymes called "restriction enzymes" in order to obtain fragments of diverse molecular weights that can be seen as bands in electrophoresis gels.
Restriction endonuclease.
Restriction endonuclease.
Restriction endonuclease.
Restriction endonuclease
A restriction enzyme will cut a DNA sequence only if it matches the specific recognition sequence of that enzyme. These enzymes are highly specific and will cleave the DNA at a particular site when the target sequence is present in the DNA molecule.
Restriction enzymes recognize specific sequences of nucleotides in DNA molecules, called recognition sites. These enzymes then bind to these sites and cut the DNA at specific points within or near the recognition site, resulting in the cleavage of the DNA molecule.
BamHI is a restriction enzyme that recognizes the specific DNA sequence "GGATCC" and cuts between the G and the A. The number of DNA fragments produced by BamHI cutting a DNA molecule depends on the number of BamHI recognition sites present in that molecule. Each recognition site will result in one additional fragment; thus, if there are n cut sites, the DNA will be divided into n+1 fragments.