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What substances are found on the human skin that react with ninhydrin resulting to stain or violet coloration on the skin?
Wiki User
∙ 11y ago
Ninhydrin (2,2-dihydroxyindane-1,3-dione) is a substance intended to discover the existence of Ammonia or anything that contains Amines. When this substance reacts with these Amines a blue-purple stain will be observed. Since, the skin contains amino acid, a blue-purple stain will be noticed.
Wiki User
∙ 11y ago
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Ninhydrin reacts with ammonia, a primary amine, or a secondary amine (amino acids have a primary or alpha amino group, except for proline which has a secondary amino group). They all turn purple/blue right away upon heating with ninhydrin.Remember that a protein has a amino terminal and a -COOH terminal. The ninhydrin will react with the amino terminal giving a very light blue or violet color (more often than not you will see no color change). Upon further heating you may notice an increase in the intensity of the blue/violet. this is due to the heat denaturing the protein, thereby exposing more -NH2 groups for the ninhydrin reagent to react with
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