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Ninhydrin reacts with ammonia, a primary amine, or a secondary amine (amino acids have a primary or alpha amino group, except for proline which has a secondary amino group). They all turn purple/blue right away upon heating with ninhydrin.
Remember that a protein has a amino terminal and a -COOH terminal. The ninhydrin will react with the amino terminal giving a very light blue or violet color (more often than not you will see no color change). Upon further heating you may notice an increase in the intensity of the blue/violet. this is due to the heat denaturing the protein, thereby exposing more -NH2 groups for the ninhydrin reagent to react with
Wiki User
∙ 16y ago
Wiki User
∙ 13y ago-Absorption Spectroscopy
The Protein Peptides absorb some blue (degradation) photons and leaves a purple color.
The intensity of the resulting color, determines the amount of Protein.
Wiki User
∙ 12y agoThis reagent reacts specifically to peptide bonds which are present in proteins. Amino acids are chained together by peptide bonds to make proteins.
An isolated amino acid simply will not react, as it is only a building block of a potential protein given the right circumstances (peptide bonds). ---> -C(=O)NH-
An amino acid on it's own just doesn't have the right chemical structure to react with biurets reagent.
Wiki User
∙ 16y agoi think that all proteins give a posotive biuret test because of the presence of peptide bonds
Wiki User
∙ 11y agobecause it take o atom from protein 2 compose NH3
Wiki User
∙ 12y agoninhydrine reacts with alpha amino acid which is present in both amino acid and protein
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What does each word represent in terms of protein synthesis?
A. DNA has the genetic sequences in the right order to make the proteins. It shows what order the amino acids have to be in to make a protein. B. mRNA is the messenger; hence m for messenger RNA. It takes a copy of the genetic sequence from the DNA and leaves the nucleus of the cell and goes to the ribosome for translation. C. tRNA is responsible for transferring the amino acids in the cytoplasm to the ribosome. Once it drops off the amino acid in the ribosome, it leaves and attaches to another for the same process. D. Amino acids are the building blocks of all living organisms. A sequence of them and put them together to form a poly-peptide.
If a urine test shows both blood and protein present what does this mean?
possible kidney stones
Infer why base substitutions in the third position are least likely to cause a change in the amino acid for which it coded.?
In figure 12.14 it shows that sometimes when you change the third base it will still code for the same amino acid. This is because since there is already 2 bases that are correct, even if the third base would change it would still code for the same amino acid.
The phylogenetic tree below shows similarities in the amino acid sequences of cytochrome-C of several organisms Which organism is most distantly related to the rhesus monkey?
The Human
How do Positive photo tropism and positive gravitropism occur?
Gravitropism is responce to Earth's gravity. If you are talking about plants then a Positive gravitropism grows towards gravity (like roots of plant). A Negative gravitroism grows away from gravity (Like the stems of plants). Hope this help :P
What color develops when ninhydrin react with amino acids?
These are the amino acids that will yield positive result to the ninhydrin test: Non-polar amino acids: Glycine, Alanine, Valine, Leucine, Isoleucine, Phenylalanine, Methionine, Tryptophan Polar Neutral Amino acids: Serine, Cysteine, Threonine, Asparagine, Glutamine, Tyrosine Polar Acidic Amino Acids: Aspartic acid and Glutamic acid Polar Basic Amino Acids: Histidine, Lysine, Arginine
What is an aminogram?
An aminogram is a diagram which shows the amino acid composition of a peptide or protein.
What is the purest form of protein?
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How much of the essential amino acids do various foods contain?
You can see a breakdown of each amino acid contained in a wide range of foods at the Nutrition Data web site:http://www.nutritiondata.com/This site shows a visual representation of all nine essential amino acids contained in a given food in a graph they call Protein Quality. Quoted from the site:----- begin quote -----The Protein Quality segment of the analysis includes another unique symbol, called a Protein Quality Indicator. Each spoke on this radial graph represents one of the nine essential amino acids. The size of each spoke is proportionate to the percentage of the optimal level for that amino acid. The amino acid with the lowest level is considered the "limiting" amino acid for that food, and determines the overall Amino Acid Score. Example: [graphic omitted -- see www.nutritiondata.com]Note: Optimal levels for amino acids are based on the following amino acid profile recommended by the Institute of Medicine's Food and Nutrition Board:Essential Amino Acid mg/g of ProteinTryptophan 7Threonine 27Isoleucine 25Leucine 55Lysine 51Methionine+Cystine 25Phenylalanine+Tyrosine 47Valine 32Histidine 18The Amino Acid Score calculated by [Nutrition Data] is a basic measure of protein quality, but it has not been adjusted for the digestibility of the protein. Protein digestibility depends on the type of protein (animal proteins are more digestible than plant proteins), as well as the method in which the food was prepared. If digestibility is accounted for, the Amino Acid Score will be somewhat lower than stated. If the Amino Acid Score is less tha[n] 100, a link is provided to "complementary" sources of protein. These are foods that are higher in the limiting amino acid(s), and/or lower in the amino acids most prevalent in the food. By combining complementary proteins, you may be able to increase the overall quality of the protein you consume. ----- end quote -----Here are some amino acid scores from the Nutrition Data web site for some example foods:* "Beef, chuck, mock tender steak, separable lean only, trimmed to 1/4" fat, USDA select, raw [Mock Tender Steak]" has an amino acid score of 154.* Canned light tuna has an amino acid score of 148.* "Turkey, fryer-roasters, light meat, meat only, raw" has an amino acid score of 145. * "Seaweed, spirulina, raw" has an amino acid score of 103.* "Game meat, elk, cooked, roasted" has an amino acid score of 96.* Pinto beans, "mature seeds, sprouted, cooked, boiled, drained, with salt", has an amino acid score of 86.* Green raw soybeans has an amino acid score of 85.* Frozen uncooked spinach has an amino acid score of 68.
Which element in the molecules of urea shows that it is formed from amino acids and not from glucose?
It has nitrogen.
Where can I purchase some amino acid supplements?
The website below you shows where you can buy your amino acid for a really great deal and the best quality also it has more info on amino acid. www.raysahelian.com/aminoacid.html
What is going on when a girl has her period and it is on and off and then she takes a pregnancy test right after that and it shows positive?
She's pregnant if the test shows positive
What does each word represent in terms of protein synthesis?
A. DNA has the genetic sequences in the right order to make the proteins. It shows what order the amino acids have to be in to make a protein. B. mRNA is the messenger; hence m for messenger RNA. It takes a copy of the genetic sequence from the DNA and leaves the nucleus of the cell and goes to the ribosome for translation. C. tRNA is responsible for transferring the amino acids in the cytoplasm to the ribosome. Once it drops off the amino acid in the ribosome, it leaves and attaches to another for the same process. D. Amino acids are the building blocks of all living organisms. A sequence of them and put them together to form a poly-peptide.
What color shows that protein is present in food?
Lilac colour.
Difference between negative health and positive health?
Negative health is a bad thing, it shows that there is regression. Positive health on the other hand, is a good thing. It shows improvement.
What does the configuration of an atom shows?
The configuration of an atom shows the arrangement of electrons, and also if the charge is positive or negative.
On a drug screen when soma test positive what class does it come under?
It shows a false positive as Benzodiazepines!!
