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How are RFLP made?
A DNA sample is broken into pieces by restriction enzymes and the resulting fragments are separated according to their lengths by gel electrophoresis. RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. But isn't as widely used now.
How can DNA from a crime scene be multiplied?
DNA from a crime scene can be multiplied using the PCR (polymerase chain reaction) technique. See the Related Link below.
What technique do researchers use to access a cell's DNA?
One can rinse the mouth with a solution and the solution is put back into the tube and add the required solution to it, then you will be able see a murky/jelly-white substances on the surface of the water, and that is the DNA which was extracted from your mouth.
What can one do with a DNA kit?
With a DNA kit you can use the swabs and a sample of your saliva to see if someone's DNA matches another person. This is often used to determine paternity of a child.
What is difference between Bac arrays and DNA arrays?
BAC (Bacterial Artificial Chromosome) arrays are a type of DNA arrays. BAC arrays are usually used for a technique called array CGH (Comparative Genomic Hybridisation) which is used to identify gross deletions or amplifications in DNA (which for example is common in cancer). DNA arrays include BAC arrays but also oligo, cDNA, and promoter arrays. Oligo and cDNA arrays are typically used for gene expression analysis (looking to see how heavily expressed each gene is). Oligo arrays can also be used for SNP (single nucleotide polymorphism) analysis. Promoter arrays are used to identify transcription factor binding sites.
How are RFLP made?
A DNA sample is broken into pieces by restriction enzymes and the resulting fragments are separated according to their lengths by gel electrophoresis. RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. But isn't as widely used now.
If you have a restriction enzyme that cuts a piece of DNA at two recognition sites how many DNA fragments would you see on a gel?
Three.To see why, cut a piece of string in two places! Of course, strictly you would not be able to see only three fragments. You would amplify the DNA before carrying out electrophoresis. That way, you would get perhaps 200 million copies of each fragment, and they would show up. Also, you would only be able to distinguish the fragments if they were different lengths. Electrophoresis separates pieces of DNA by length.
How can DNA from a crime scene be multiplied?
DNA from a crime scene can be multiplied using the PCR (polymerase chain reaction) technique. See the Related Link below.
Why is gel electrophoresis important?
To separate and analyze DNA fragments and protein fragments by weight. If you have digested some bacterial DNA, for instance, then you can tell by running the fragmented DNA in the gel whether you have digested the correct base length.
How does DNA fingerprinting work?
since every one has there own unique DNA, the police can then get samples of it and match that DNA with a record of all other Dana's and see if it matches with one . So they are technically match making.
What are the functions of the loading dye in electrophoresis how can DNA be prepared for visualization?
First, loading dye is meant to help weigh down the DNA solution, so that it can sink into the bottom of the wells and not float in the buffer solution.Second, two different types of loading dye are used in electrophoresis. One of them moves more quickly than most of your DNA fragments, and the other moves more slowly, helping you determine the relative position of your DNA, as it should be in between the two bars or dye. This also tells you when to stop electrophoresis so that your DNA does not fall out of your agarose gel.Note that loading dye does not bind to the DNA itself and does not allow you to see the bars of DNA usually seen in a complete DNA fingerprint.
How is DNA used?
to tell people apart and to see there difference's and alike and dislike.
What technique do researchers use to access a cell's DNA?
One can rinse the mouth with a solution and the solution is put back into the tube and add the required solution to it, then you will be able see a murky/jelly-white substances on the surface of the water, and that is the DNA which was extracted from your mouth.
Why cant you see DNA within a DNA?
Because the purpose of this lab is to extract DNA from a variety of cells and see DNA
What can one do with a DNA kit?
With a DNA kit you can use the swabs and a sample of your saliva to see if someone's DNA matches another person. This is often used to determine paternity of a child.
What is difference between Bac arrays and DNA arrays?
BAC (Bacterial Artificial Chromosome) arrays are a type of DNA arrays. BAC arrays are usually used for a technique called array CGH (Comparative Genomic Hybridisation) which is used to identify gross deletions or amplifications in DNA (which for example is common in cancer). DNA arrays include BAC arrays but also oligo, cDNA, and promoter arrays. Oligo and cDNA arrays are typically used for gene expression analysis (looking to see how heavily expressed each gene is). Oligo arrays can also be used for SNP (single nucleotide polymorphism) analysis. Promoter arrays are used to identify transcription factor binding sites.
Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
