0
The purpose is to select for salt-tolerant microbes. Most species of microbes can't grow in such a salty environment.
The only genus that can culture on an MSA plate is Staphylococcus.
The only species of Staphylococcus that can ferment Mannitol is Staph. Aureus. If the plate turns yellow, you know that Mannitol was fermented into an acid, which only Staph. Aureus can do.
If there is a little growth (aka the culture is red) then it's still most likely a species of Staph such as Staph. epidermidis.
If there is no growth, as in E. coli, then there will be no culture and no change in color.
What else can I help you with?
Can enterobacter cloacae grow on mannitol salt agar?
Yes, Enterobacter cloacae typically cannot grow on mannitol salt agar because it does not ferment mannitol. Mannitol salt agar is selective for salt-tolerant Staphylococci species that can ferment mannitol, producing acid and turning the agar yellow.
Does bacillus subtilis ferment acid from mannitol salt agar?
Bacillus subtilis typically does not ferment acid from mannitol salt agar as it lacks the enzymes needed for fermentation. Mannitol salt agar is mostly used to differentiate Staphylococcus species, which are capable of fermenting mannitol.
What is the purpose for adding salt to mannitol salt agar?
High concentrations of salt inhibit the growth of non-halophilic organisms but allow for the growth of bacteria from the genus Staphylococci.
HOW do mannitol salt hook up to glycolysis?
Mannitol is not a substrate for glycolisis
What reaction does the enzyme involved in Mannitol-salt agar test mediate?
Mannitol salt agar is selective for gram positive bacteria, and differential for mannitol fermenters. Phenol red is the indicator containing the enzyme mannitol.
What ingredient makes mannitol salt selective?
the salt content
Purpose of high salt concentration in the mannitol salt agar medium?
Mannitol salt agar (MSA) contains high levels of salt because it inhibits the growth of most bacteria. This makes it an excellent medium to test for Staphylococci and Micrococcaceae as they are tolerant of high levels of NaCl.
Which ingredient in mannitol salt agar supplies carbon?
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
What organisms do not grow on msa and mannitol?
Organisms that do not grow on Mannitol Salt Agar (MSA) are non-halophilic bacteria that are unable to tolerate high salt concentrations. Mannitol-fermenting bacteria are organisms that can ferment mannitol and grow on MSA, while non-fermenting bacteria will not grow.
Can B subtilis grow on MSA?
No, Bacillus subtilis cannot grow on Mannitol Salt Agar (MSA) as it cannot ferment mannitol and does not tolerate the high salt concentration in MSA. MSA is selective for Staphylococcus species that can ferment mannitol.
Can salmonella typhimurium grow on mannitol salt agar?
No, Salmonella Typhimurium cannot grow on Mannitol Salt Agar because it is a selective and differential media primarily used for isolating and differentiating Staphylococcus species based on their ability to ferment mannitol. Salmonella Typhimurium is not a mannitol fermenter, so it will not grow on this agar.
Why is mannitol salt considered to be a differential media?
Mannitol salt agar is used for the isolation of staphylococci which is found normally on skin (S. aureus). The selectivity is obtained by the high salt concentration that inhibits growth of many groups of bacteria.
