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Mannitol salt agar (MSA) contains high levels of salt because it inhibits the growth of most bacteria. This makes it an excellent medium to test for Staphylococci and Micrococcaceae as they are tolerant of high levels of NaCl.

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Is mannitol salt agar complex or synthetic?

Mannitol Salt Agar Complex is a synthetic medium because it is prepared from pure chemical substances and the exact composition of the medium is known.


Is Mannitol salt agar a selective or a differential medium?

Both


What is MSA agar?

Mannitol salt agar or MSA is a commonly used growth medium in microbiology. It contains a high concentration (~7.5%-10%) of salt (NaCl), making it selective for Staphylococci (and Micrococcaceae) since this level of NaCl is inhibitory to most other bacteria.


Which ingredient supplies carbon in the mannitol salt agar?

mannitol is a type of sugar, so it supplies the carbon in the MSA medium


Which ingredient in mannitol salt agar supplies carbon?

mannitol is a type of sugar, so it supplies the carbon in the MSA medium


How do mannitol-salt agar tests work?

Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.


Can enterobacter cloacae grow on mannitol salt agar?

Yes, Enterobacter cloacae typically cannot grow on mannitol salt agar because it does not ferment mannitol. Mannitol salt agar is selective for salt-tolerant Staphylococci species that can ferment mannitol, producing acid and turning the agar yellow.


What selective agent found in Mannitol Salt Agar?

Mannitol Salt Agar typically contains a high concentration of salt, which acts as the selective agent. This high salt concentration provides an environment that restricts the growth of many organisms, allowing only salt-tolerant bacteria such as Staphylococcus species to grow on the agar plate.


Does E. faecalis test positive for MSA agar test?

E. faecalis typically does not ferment mannitol, so it does not grow well on mannitol salt agar (MSA) and would not test positive on this medium. MSA is selective for organisms that can ferment mannitol, such as Staphylococcus species.


Does bacillus subtilis ferment acid from mannitol salt agar?

Bacillus subtilis typically does not ferment acid from mannitol salt agar as it lacks the enzymes needed for fermentation. Mannitol salt agar is mostly used to differentiate Staphylococcus species, which are capable of fermenting mannitol.


Does Saccharomyces cerevisiae ferment mannitol?

Mannitol salt agar inoculated with Micrococcus luteusshowing no fermentation of mannitol (pink medium). The colonies show a yellow pigment which is characteristic of M. luteus.


What concentration of agar is used in a semisolid medium for motility How does that compare to a typical solid medium?

A concentration of 0.5-0.7% agar is typically used in semisolid medium for motility. This is lower than the typical 1.5-2% agar concentration used in solid medium, allowing motile bacteria to move more easily through the softer semisolid medium.