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restriction endonucleases

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Q: What type of the enzyme that cuts the bonds of DNA molecule at the origin of replication?
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In order to replicate where do DNA molecules separate?

At the hydrogen bonds between bases with the help of the enzyme helicase.


What enzyme breaks hydrogen bonds between nitrogen bases?

Helicase and RNA polymerase separate DNA strands by breaking the hydrogen bonds between complementary bases.Helicase parts the strands of DNA during DNA replication, and RNA polymerase parts them during transcription.The enzyme that separates DNA in called DNA helicases. There are two of them that work away from the origin of replication, creating in "bubble" in the DNA molecule. For eukaryotes, there would be several origins of replication but in prokaryotes, there is only one origin of replication.


In the replication of DNA what kind of molecule bonds with an unpaired cytosine mucleotide?

In the replication of DNA Thymine bonds with cytosine.


What enzyme is responsible for unwinding the DNA molecule for replication?

DNA Helicase is the enzyme responsible for unzipping DNA before replication occurs.


Is enzyme organic or inorganic?

An enzyme is a protein and has many carbon hydrogen bonds, so it is an organic molecule.


Which enzyme assembles the complemetary nucleotide base during replication?

It's the DNA polymerase that catalyzes the formation of phosphodiester bonds between the nucleotides during replication.


What breaks the bonds between the nitrogen bases of the DNA molecule?

Your answer is "Helicase". This is the enzyme responsible for the unzipping of the DNA molecule, or in other words, the breakage of the bonds of its nitrogen bases.


During replication what is the function of the enzyme DNA polymerase?

DNA polymerase are enzymes that form bonds between nucleotides during replication.


DNA replication results in two identical DNA molecules. What role do DNA helicases have in DNA replication?

DNA helicases break the hydrogen bonds in the DNA molecule


What kind of bonds hold the substrate in the active site of the enzyme?

The bonds that hold the the substrate to the active site are hydrogen bonds. These are relatively weak bonds.


What kind of molecule is EcoR1?

EcoR1 is a restriction enzyme (endonuclease), which splits the phosphodiester bonds of the backbone of DNA.


Which enzyme is responsible for creating the covalent bonds that connect the sugar phosphate back bone of the new DNA molecules?

An enzyme called HELICASE breaks the sugar to phosphate bonds in DNA strands to initiate DNA replication and DNA transcription.