In Griffith's experiment 1 he injected the mouse with live R cells.
It was not till Griffiths experiment 2 did he injest the mouse with live S cells.
Griffith's experiments involved injecting mice with different strains of Streptococcus pneumoniae. He observed that mice injected with the heat-killed virulent strain survived, while those injected with live non-virulent strains did not. Remarkably, when he mixed heat-killed virulent bacteria with live non-virulent bacteria, the mice developed pneumonia and died. This indicated that some "transforming principle" from the dead virulent bacteria was taken up by the live non-virulent bacteria, suggesting that DNA was responsible for the trait of virulence.
The injected microorganisms in a vaccine may have the same antigens as the live pathogen, but they are weakened or killed to prevent disease. This allows the immune system to recognize and build immunity against these antigens without causing illness.
Griffith's experiments demonstrated that a hereditary factor was involved in bacterial transformation through the use of two strains of Streptococcus pneumoniae: a virulent smooth strain and a non-virulent rough strain. When he injected mice with heat-killed smooth bacteria mixed with live rough bacteria, the mice developed pneumonia and died, indicating that the rough bacteria had somehow transformed into the virulent smooth form. This transformation suggested the presence of a "transforming principle," which later researchers identified as DNA, thus showing that genetic information could be transferred between bacteria.
Oswald Avery and his associates followed several key steps of the scientific method in their transformation experiments. They began by making observations about the effects of heat-killed pathogenic bacteria on non-pathogenic strains. They formed a hypothesis that a substance from the heat-killed bacteria was responsible for transforming the non-pathogenic bacteria into pathogenic forms. Through systematic experimentation, they isolated and purified DNA, demonstrating that it was the transforming principle, thus supporting their hypothesis with empirical evidence.
The mice died. This was a fundamental experiment in the history of genetics and helped to establish that DNA was the information and gene carrier for all life. To understand it a little better it's important to know that the heat killed (virulent) bacteria killed the mice when it was living. And the non-virulent did not. But when combined, the non-virulent absorbed some of the DNA from the virulent and was able to make a protective coat that shielded it from the animals immune system... allowing it to kill the mice.
To find out, he took a culture of these cells, heated the bacteria to kill them, and injected the heat-killed bacteria into the mice.
To find out, he took a culture of these cells, heated the bacteria to kill them, and injected the heat-killed bacteria into the mice.
To find out, he took a culture of these cells, heated the bacteria to kill them, and injected the heat-killed bacteria into the mice. The Mice survived, suggesting that the cause of pneumonia was not a chemical poison released by the disease-causing bacteria.
Boiling the meat broth killed any existing microorganisms.
Boiling the meat broth killed any existing microorganisms.
He killed them!
Griffith's experiments involved injecting mice with different strains of Streptococcus pneumoniae. He observed that mice injected with the heat-killed virulent strain survived, while those injected with live non-virulent strains did not. Remarkably, when he mixed heat-killed virulent bacteria with live non-virulent bacteria, the mice developed pneumonia and died. This indicated that some "transforming principle" from the dead virulent bacteria was taken up by the live non-virulent bacteria, suggesting that DNA was responsible for the trait of virulence.
S Strain ---> inject into mice ---> Mice Die R Strain ---> inject into mice ---> Mice Live Griffith was able to kill bacteria by heating them. He observed that heat-killed S Strain bacteria injected into mice did not kill them. When he S Strain (heat Killed) ---> Injected into mice ---> Mice Live S Strain ( heat Killed) + R Strain (Live) ---> Injected into mice ---> Mice Die
It depends - on how much venom is injected, and how far away you are from help !
around 30 or so for experiments!
The term "transformation" is appropriate for Griffiths' observations because it describes the process by which genetic material from one organism can change the characteristics of another organism. In his classic experiments with Streptococcus pneumoniae, Griffith demonstrated that non-virulent bacteria could become virulent when exposed to heat-killed virulent strains, indicating a transfer of genetic information. This process of acquiring new traits through the uptake of foreign DNA is fundamentally what transformation entails in a biological context.
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