You need to specify what the changes were a result of if you make it possible to answer your question. There were many changes in ancient Rome over the 1,200 years of her history and they were the result of many different things.
If the changes seen under the microscope don't point to any particular diagnosis, the biopsy result will be nonspecific.
Athena was last seen in Rome
Physical change, if it changes in a way to be seen, or chemical change, which if the change can not be seen
the rome captive the jews
civil war
It destroyed the walls
Most chemical changes happen between molecules, so they are unseen.
Sistine Chapel in The Vatican.
what changes happened at the time as a result of this scientists work
Rome won
Rome.
The dependent variable changes as a result of a change in the independent variable. (Hence the name "dependent")