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What would happen if the electrodes were plugged into the wrong outlet during gel electrophoresis?
JessieSpradlinggp762...
DNA migrates from the black (negative) terminal to the red (positve) if you place your DNA in the wells adjacent to the red terminal in would in a short time migrate off the end of your gel into the running buffer. Most people who run DNA gels have done this at least once.
Wiki User
∙ 15y ago
Wiki User
∙ 11y agoDNA has a negative charge. When you run a gel, you start with the wells containing DNA closest to the negative electrode. Positive attracts negative so the DNA migrates through the gel towards the positive electrode. If you were to start with the DNA wells near to the positive electrode, the DNA would still move towards the positive electrode - off your gel into your buffer, which would give you no data and buffer solution full of DNA. So you always start with the wells at the negative end so the negatively charged DNA moves through the gel towards the positive electrode.
Wiki User
∙ 9y agoNothing
maybe a spark but it wouldn't hurt a fly
Wiki User
∙ 10y agowill cause infertility..
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What has to happen before you can run DNA through gel electrophoresis?
Pcr
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What has to happen before you can run DNA through gel electrophoresis?
Pcr
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This is a really bad idea. The current will double and bad things can happen.
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in what situation might it be better to position the electrodes on the upper arms and thighs rather than lower on the arms and legs
