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Five I's
1. Inoculation: The sample is placed into a container of sterile medium that provides microbes with the appropriate nutrients to sustain growth.
2. Incubation: An incubator can be used to adjust the proper growth conditions of a sample.
3. Isolation: The end result of inoculation and incubation is isolation of the microbe.
4. Inspection: The cultures are observed for obvious growth characteristics that could be useful in analyzing the specimen contents.
5. Identification: Determine the type of microbe, usually to the level of species.
Wiki User
∙ 12y ago
Wiki User
∙ 11y agoIt's mostly a matter of extent. Inoculation is simply exposure. Contamination is not so controlled and usually far more harmful.
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