If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
This is because of the refractive propoties of the immersion oil. The oil works as a sort of secondary lens, magnifying the specimin in addition to the lens alresdy present.
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
In light microscopy, oil immersion is a technique used to increase the resolution of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.
The purpose to use oil is to make it clear in the microscope. Oil is the quintessential "tool" to make oil immersion magnification possible. Oil has the same refractive index as glass, therefore the light is not bent by the air that would otherwise be in the oil's place.
100x lens is used with oil immersion
Oil immersion is a technique used to increase the resolution of a microscope.
Cedar wood oil
If you are using the oil immersion objective on a microscope, you must use oil to increase the resolution of the lens. These lens are used at very high magnification.
oil immersion
immersion oil
the hanging drop procedure uses a thicker slide, which could crack if the oil immersion lens is used. My bio professor worte: hanging drop slides asre too thick to use under oil.
When using the oil immersion objective, the oil has the same refractive index as the glass. So it is like an extension of the lens. Water does not have the same refraction index as glass, so the image would not be as clear.
So that you do not scratch the lens because at 100x magnification it is very very close to the slide.
This is because of the refractive propoties of the immersion oil. The oil works as a sort of secondary lens, magnifying the specimin in addition to the lens alresdy present.
Not only does oil immersion increase the microscopic resolution of a specimen, it is also transparent. This allows for optimal microscopy views.
In light microscopy, oil immersion is a technique used to increase the resolution of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.